中国人群外周血MLPH基因高甲基化与冠心病相关

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Abstract： ObjectiveToinvestigatetheassociationbetweenmethylationlevelsoftumorsuppressngsubtransferablecandidate1 （TSSC1）and melanophilin （MLPH） genes in peripheral blood and coronary heart disease （CHD）in Chinese population. MethodsThiscase-controlstudywasconductedin86CHDpatientsand95healthyindividuals，whosemethylationlevelsof TSSC1 and MLPH genes in peripheral blood were determined using mass spectrometry. Mann-Whitney U test was used to comparethe methylationlevels indiferentsubgroups.Thecorrelationof TsSC1and MLPHgene methylation levels withage andgender were evaluated using Spearman correlationcoeffcientand contingency coefficient，respectively.Results Comparedwiththehealthyindividuals，theCHDpatientsshowedasignificantcorrelationbetweenMLPHhypermethylation and myocardial infarction （MI） （MLPH_CpG_2.7： P=0.045; MLPH_CpG_3/cg06639874： P=0.049; MLPH_CpG_5： P=0019），and thiscorelationwasevenstrongerinindividualsbelow65yearsofage（MLPHCpG_2.7：P-0014;MLPH_CpG_4：P-0001）and inmale subjects （MLPH_CpG_2.7：P=0.004;MLPH_CpG_3/cg06639874： P=0.04）.The methylation levelof TsSC1 gene in peripheral blood was notfound tocorrelate with CHDorits subtypes.Conclusion Our findings suggesta corelationof MLPH hypermethylation inperipheralblood withCHDandMI inChinese population，especiallyinindividuals below 65yearsand in male individuals.

Keywords： coronaryheartdisease; TssC1; MLPH; DNAmethylation; peripheral blood

冠心病（CHD）是全球死亡率最高的疾病之一[]根据《中国心血管健康与疾病报告2021摘要》，我国约有1139万CHD患者，给社会带来了巨大的经济负担[2]。（剩余13735字）