槐花通过抑制PI3K/AKT通路减轻炎症反应治疗银屑病

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Abstract：ObjectiveToexplorethetherapeuticmechanismofFlosSophorae（FS）fortreatmentofpsoriasis.MethodsTheactive ingredients，argetsadpsoasis-relateddseasetargetsofSwereoaiedfroGneCardsO，iead StringdatabasesandCytoscape3.8.0softwarewasusedtoconstructthe"FS-activeingredient-keytarget-signalingpathway psoriasis"network.GOandKEGGenrichmentanalysesofthekeytargetswereconducted，andmoleculardockingwas performedusingDiscoveryStudio2019.InaBALB/cmousemodelofimiquimod-inducedpsoriasis，theefectsofvaseline，FS at high，mediumandlow doses （3.0o，1.50and 0.75g/kg， respectively） and a positive drug， given 1 week before and during modeling，wereevaatedonbodyweightchanges，spleencoeficient，psoiasisaeaandseverityindex （PASI）scoeadkin pathological changes.Phosphorylation levels of PI3K and AKT proteins were detected using immunohistochemistry and Westernblotting.ResultsA total of10activecomponentsand 110key targetswere screened.GOandKEGG pathway enrichmentanalysissuggestedthatFSimprovedpsoriasisprimarilythrough thePI3K/AKT，TNF，and IL-17signaling pathways.Moleculardockingshowed thatboth quercetinandkaempferolcould spontaneouslybindtoAKT1，TNFandother sites.Inthemousemodelofpsoriasis，treatmentwithlow-doseFSsignificantlyimprovedepidermalthickeningincreased bodyweight，loweredPASIscoreandreducedphosphorylationlevelsofPI3KandAKproteins.ConclusionThetapeutic mechanismofFSforpsoriasisinvolvesmultiplecomponents，targets，andpathwaysthat mediatetheinhibitionof the phosphorylation levels of PI3Kand AKTproteins to suppress the activation of the PI3K/AKT signaling pathway. Keywords： FlosSophorae; psoriasis; network pharmacology; molecular docking; PI3K/AKT signaling pathway

银屑病是一种常见的慢性复发性皮肤病，发病率较高，易于复发，病程较长，对患者的身体健康和精神影响大，是当前皮肤病领域内重点研究的疾病之一[。（剩余13623字）