铁死亡相关基因对溃疡性结肠炎具有诊断预测价值

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Abstract：ObjectiveToexplorethevalueofferroptose-related genesinthediagnosisand predictionofulcerativecolis（UC）. Methods We used UC dataset from the GEO database to screen for diferentially expressed genes （DEGs）in UC.The DEGs related to feroptositis were screened from the FerrDbdatabase and their functions wereanalyzed.The hub genes were identifiedbyconstructingtheprotein-proteininteractionnetwork（PP），thediferencesinimmuneinfiltrationlevelsbetween UCandthecontrol group were evaluatedusing CIBERSORT，andthediagnostic values ofthe hub genes forUCwere verified byusingthetrainingset.InamousemodelofUC，weexaminedtheexpressionlevelsof thehubgenesinthecolontisuesof the miceusingreal-timefluorescencequantitativePCR（qPCR）.Results Weidentifedatotalof76DEGsrelatedtoferotosis. Functionalenrichmentanalysisshowedthatthesegenesweresignificantlyenrichedinferoptosisandhypoxiapathways.The PPInetwork identified10ubgenes，and9of them wereighlyexpressedin UC.Analysisofimmunecellinfiltrationshowed that27cell typeswere significantly increased in UC （P<0.05） ，andthe immune checkpoints-related geneshad the strongest correlationwith the hub gene PPARG （P<0.05） .Verificationanalysisusingthetrainingsetshowed thatP4HB，PPARGand STAT3 had the best predictive value for UC （P<0.05） . In the UC mouse model， the expression of PPARG was significantly decreasedandtheexpressonsofP4HBand STAT3 were significantlyincreased inthecolontissuesofthe miceasompared with the normal mice.Conclusion Ferroptose-related genes havesignificant valuefordiagnosisand predictionof UC.

Keywords：ulcerativecolitis; ferroptosis;immune infiltration;PPARG;Tcells

溃疡性结肠炎（UC是一种复杂的慢性炎症性肠道疾病，具有反复发作和缓解的特征。（剩余14831字）