纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发及生理特性的影响

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中图分类号：S541.9 文献标识码：A 文章编号：1007-0435（2026）04-1534-12

Abstract：Drought stressinhibits seed germination of alfalfa（Medicago satiua L.）and reduces its yield.This research studied the efects of cerium oxide nanoparticle priming on seed germination and physiological characteristics of alfalfa under drought stress.Alfalfa seeds were used as materials and primed with different concentrations of cerium oxide nanoparticle （ CeO2NPs ）solutions（0，50，100，150， 200mg⋅L-1; ）inthedark for 24hours. Drought stress was simulated with 15% PEG to explore the mitigating effect of CeO2NPs on seed germination. The results showed that 15% PEG significantly inhibited seed germination，while priming with 150mg-L-1 CeO2NPs increased the germination rate to 77% （ P<0.05 ），whichwas 5% higher than the nonprimed treatment. It also shortened the average germination time，increased the germination index，and enhanced the fresh weight of germinated seedlings. At the physiological level，the 150mg⋅L-1 （204号 CeO2NPs priming treatment increased the activities of α-amylase and β -amylase，and significantly reduced the contents of reducing sugar （ ⋅P<0.05） ；the 100mg⋅L-1 treatment significantly improved the activity of antioxidant enzymes；the 50mg⋅L-1 treatment effectively reduced the ROS content；all concentrations of CeO2NPs priming treatments significantly decreased the malondialdehyde content （ .P<0.05 ）and the proline content，and increased the soluble protein content as osmotic adjustment substances. Comprehensive analysis indicated that 150mg.L-1 CeO2NPs priming had the best mitigating effect on alfalfa seed germination under drought stress.

Key words： Alfalfa; Drought stress;Cerium Oxide Nanoparticles;Seed priming;Seed germination

紫花苜蓿（MedicagosatiuaL.）作为“牧草之王”，因产量高、营养丰富而在畜牧业中占据重要地位，但其多年生高耗水特性使其对干旱胁迫极为敏感[1]。（剩余19690字）