杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、 鉴定及除草潜力评价

打开文本图片集

中图分类号：S451 文献标识码：A 文章编号：1007-0435（2026）04-1196-09

doi：10.11733/j.issn.1007-0435.2026.04.006

Abstract：To screen biocontrol strains with high herbicidal potential，the pathogenic fungus JZ-28 was isolated fromnaturally infected leaves of Viola philippica in Qinghai Province. Through morphological characterization and multi-gene（ITS， EF-1α ，LSU） phylogenetic analysis，the isolate was identified as Clonostachys rosea. Pathogenicity assays demonstrated potent herbicidal activity of strain JZ-28，inducing chlorosis in detached leaves of four dominant broadleaf weed species from the Qinghai-Xizang Plateau（Malua verticillata，Elsholtzia densa，Senecio uulgaris，and Chenopodium album） within 7 days post-inoculation （dpi）. Disease incidence rates in whole-plant assays reached 83% ， 95% ， 100% ，and 70% ，respectively. Safety evaluation on six major crops and three cool-season vegetables in Qinghai revealed that JZ-28 was safe for four crops： Zea mays，Vicia faba，Pisum satioum， Brasica napus，and three vegetables： Cucumis satius， Solanum lycopersicum，and

Solanum melongena.Lightly Susceptible Was observed in highland barley Hordeum uulgareand Triticum aestiuum.These findings indicated JZ-28’s potential as a broad-spectrum bioherbicide against broadleaf weeds in multiple cropping systems. Culture optimization identified sucrose and soybean meal as optimal carbon and nitrogen sources for enhancing mycelial growth and sporulation. This study provided the first evidence of Clonostachys rosea's bioherbicide potential inalpine ecosystems，addressing a critical research gap in microbial herbicide development and offering sustainable weed management solutions for fragile environments.

Key words：Weed control; Clonostachys rosea;Microbial herbicides

农田杂草是农业生产中的重要制约因素，我国每年因杂草危害导致的农作物产量损失约 10% ～15% ，联合国世界粮农组织FAO统计数据显示，全世界每年因杂草带来的损失高达210亿美元1。（剩余13836字）