紫花首蓿MsHSP18.2基因的克隆与功能分析

打开文本图片集

中图分类号：S541.9 文献标识码：A 文章编号：1007-0435（2026）04-1158-10

doi：10.11733/j.issn.1007-0435.2026.04.003

Abstract：Smallheat shock proteins （Small heat shock proteins， sHSPs）are a class of stress-induced proteins widely present in plants，play crucial roles in plant growth，development，and response to drought and salt stresses.To investigate the function of the MsHSP18.2 gene in Medicago satia，bioinformatics tools were employed to predict and analyze its theoretical properties，structure，and function.Plants were treated with methyl jasmonate（MeJA），salicylic acid （SA），abscisic acid（ABA），indole-3-acetic acid （IAA），gibberelin （GA），and cytokinin （6-BA），as well as subjected to drought and salt stress.Gene expression levels were analyzed by qRT-PCR at O，O.5，1，3，6，9，and 12 hours post-treatment. Transcriptional autoactivation was examined by using a yeast expresson system.The stress resistance function was verified using a heterologous yeast system.The results showed that this protein has a molecular weight of 18.18 KDa and a theoretical iso electric pointof5.55，exhibiting relatively high instability（instability index：54.67）.Itssecondarystructure was predominantly composed of random coils. Expression analysis demonstrated that MsHSP18.2 was upregulated under drought and salt stress treatments and displayed diferential responses to various plant hormones. Yeast autoactivation assays confirmed its autoactivation activity. Heterologous overexpression of MsHSP18. 2 gene significantly enhanced the salt tolerance （NaCl） and osmotic stress （PEG） tolerance of yeast cels.

Key Words：MsHSP18.2;Medicago satiua;Bioinformatics analysis;Expresson analysis;Self-activationdetection

热休克蛋白（Heatshockproteins，HSPs），作为一类高度保守的蛋白质分子，在生物界中普遍存在[1-2]，在生物体受到外界刺激或胁迫时上调表达，协助细胞内蛋白质正确构象和分子组装，发挥分子伴侣作用[3]。（剩余15931字）