南疆沙区油莎豆根际耐盐促生菌株的筛选

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中图分类号：S548 文献标识码：A 文章编号：1007-0435（2026）04-1181-15

Abstract：To screen salt-tolerant and growth-promoting bacterial strains adapted to the extreme environment of Southern Xinjiang from the rhizosphere soil of Cyperus esculentus L.and enrich the strain resource bank for saline-alkali sandy soils in Xinjiang，this study employed the targeted screening medium culture method，shake flask culture method，and16S rDNA identification method’ to isolate salt-tolerant and growth-promoting strains，determine their growth-promoting characteristics，and finally further verify the growth-promoting effects of these strains through Cyperus esculentus seed germination tests and potted seedling experiments. A total of 1O salt-tolerant and growth-promoting strains were obtained，which belonged to the genera Bacillus， Vreelandella，Exiguobacterium，and Bisbaumanmia，with their salt tolerance thresholds ranging from 4% to 8% NaCl.Among these strains，strain Y-38 （Exiguobacterium acetylicum） exhibited prominent inorganic phosphorus-solubilizing ability （0.65mg⋅L-1） ，strainY-35（ E xiguobacterium mexicanum） showed the highest production of indole-3-acetic acid （IAA）（6 4.35mg⋅L-1） ，and strain Y-46 （Bisbaumannia pacifica） could tolerate 8% NaCl.In the seed germination experiment，the germination rate of the Y-38 treatment group reached 82.00% ，which was significantly 26.89% higher than the control group （ P<0.05 ）；in the potted experiment， growth indicators of the Y-38 treatment group，including plant height （39.17cm） ），leafwidth 0 （5.22mm） ，and stem diameter （5.52mm ），were all significantly higher than those of the Y-46 and Y-35 treatment groups（ P<0.05 . The comprehensive ranking of the growth-promoting effects of the three strains was Y-38>Y-46>Y-35 . Ultimately，the high salt tolerance and growth-promoting function of strain Y-38 （Exiguobacterium acetylicum） was confirmed，providing a bacterial strain resources for the improvement of saline-alkali soils.

Key Words：Cyperus esculentus L.;Rhizosphere growth-promoting bacteria;Salt-tolerant bacteria;Isolation and identification

南疆作为我国重要的战略屏障和向西开放的关键门户，其生态安全与社会稳定是国家战略实施的基石[1]。（剩余26928字）