药用植物卷叶黄精根际解磷细菌的筛选鉴定及培养条件的优化

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关键词：卷叶黄精；根际细菌；解磷能力；培养条件中图分类号：S567.239 文献标识码：A 文章编号：1007-0435（2026）04-1510-14

Abstract：To addressthe current status of soil phosphorus deficiency，this study aimed to develop novel，safe， and eficient microbial fertilizers.Polygonatum cirrhifolium from Tibet was used as the test material，and the streak plate method was employed to isolate and screen phosphorus-solubilizing strains.Their phosphorus-solubilizing capacity was determined，and the culture conditions were investigated. The results showed that atotal of 9 phosphorus-solubilizing bacterial strains were isolated. Among them，5 strains with a phosphate solubilization index （PSI）>2.0 were screened，with phosphorus-solubilizing capacities ranging from 29. 6O3 to 41. 730 mg⋅L-1 . The strains were identified as follows： JYHJ-1 as Pseud-omonas yamanorum， JYHJ-2 as Pseudomonas koreensis，JYHJ-3 as Serratia proteamaculans， JYHJ-4 as Pseudomonas azotoformans，and JYHJ-5 as Microbacterium oxydans. Strain JYHJ-3 exhibited the strongest phosphorus-solubilizing ability，and it could secrete 7 types of organic acids as wellas possesscapabilities such as siderophore production，potassium solubilization，nitrogen fixation，and indole-3-acetic acid（IAA）production.In the single-factor experiments，the optimal phosphorus-solubilizing eficiency was achieved when lactose was used as the carbon source，ammonium acetate as the nitrogen source，initial pH was 5.5， inoculation volume was 2% ，and NaCl concentration was 1% . Based on the single-factor experiments，response surface methodology （RSM） was applied to optimize the initial pH ，inoculation volume，and NaCl concentration. The final optimal culture conditions were determined as follows： lactose as the carbon source，ammonium acetate as the nitrogen source，initial pH5.5 ， inoculation volume 3% ，and NaCl concentration 1% . In conclusion，the rhizosphere of Polygonatum cirrhifolium was rich in phosphorus-solubilizing bacterial resources.This study provided strain resources and a theoretical basis for the sustainable cultivation of medicinal Polygonatum cirrhifolium and the development of microbial fertilizers.

Key Words：Polygonatum cirrhifolium;Rhizosphere bacteria;Phosphate-solubilizing capacity; Fermentation conditions

磷是植物生长发育不可或缺的关键营养元素，参与光合作用、能量代谢、细胞分裂等重要生理过程[1]，其有效性直接制约着生态系统生产力与农业可持续发展。（剩余25232字）