基于miR-146α调控TLR4/NALP3信号通路探讨痛风立安胶囊的抗炎机制

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【中图分类号】R285.5 【文献标志码】A 【文章编号】1007-8517（2025）12-0030-05

DOI： 10.3969/j . issn.1007-8517.2025.12. zgmzmjyzz202512008

Abstract：ObjectiveThisstudyaims toinvestigate theanti-inflammatoryefectofTongfengLianCapsuleonacutegoutyarthritis anditsunderlying mechanisms.MethodsArandomizedcontroleddesignwasadopted，andSPF-graderatsweredividedintofour groups：model group，colchicine group，Tongfeng LianCapsulegroup，andnormalcontrolgroup.Theacutegoutyarthritis odelwas inducedby monosodiumurate（MSU）crystals，andthecoespondingdrugsornormalsalinewereadministered.Jointswellngindex was measured at 1h before modeling and at 2 h， 4h ！ 6h ，and 12h after modeling.After 3 days，samples were collected to detect the levels of IL-1β ， TNF-α ，PGE2，TGF-β1，and 1L-8 in serum，as well as the expression levels of NALP3，TLR4，and NF- κB （20 p65 proteins andmiRNA-146αmRNA insynovial tssue.ResultsCompared with thenormalcontrolgroup，ratsinthemodel group showedsignificant inflammatoryresponses，with significantlyincreasedankle jointswellngrate，serum inflammatoryfactors （IL-1β TNF-α ，PGE2，TGF-β1，IL -8），and expression levels of NALP3，TLR4，and NF - κκκ p65 proteins in synovial tissue. The level of miRNA -146α in synovial tissue wassignificantly decreased.Compared with the model group，theankle joint swellingrate，serum inflammatory factors（ IL-1β ， TNF-α ，PGE2，TGF-β1，IL-8），and expresson levels of NALP3 and TLR4 proteins in synovial tissue were significantly reduced in the Tongfeng Lian Capsule group，while miRNA -146α in synovial tissue was significantly upregulated.There was no significant difference in NF- κB p65 protein expression. Conclusion Tongfeng Lian Capsule exhibits a significant anti-inflammatoryefectonAGAanditsmechanismmayinvolveinibitinginfammatoryresponsesthroughtheregulationofmiNA -146α and the TLR4/NALP3 signaling pathway.This providesa scientific basis fortheclinical aplicationof Tongfeng LianCapsule.

Key Words：Tongfeng Lian Capsule;Gouty Arthritis；Inflammatory

痛风性关节炎（goutyarthritis，GA，简称痛风）是一种常见的关节炎症，其发病常伴随不良的饮食习惯和生活方式1]，具有高发性、致残性和反复性等特点。（剩余6516字）