水晶香柚茎段离体诱导培养条件初探

打开文本图片集

中图分类号：S666.3 文献标识码：A

Abstract：【Objective】To explore the suitable culture conditions virus-fre tissuecultureseedlings pomelo，inorder toscreenthe method eficientinductionadventitious budspomelo，providereferencefortheutilizationdevelopment pomelo germplasm resources sustainable healthy development in .【Methods】Adult Shui Jing Xiang pomelo branches were disinfected with varying NaClOconcentrations"Bycomparing thedata，the appropriate explant disinfection methods，coection season，themostappropriatehormoneratiorotstock seed disinfectionmethods fortissueculture wereselected.Micro bud grafting experiments werecarriedouttoanalyze thesurvival status microbud grafting.【Results】 The optimal disinfection method for the explants Shui Jing Xiang pomelo was 75% alcohol +5% NaClO disinfection for 20min twice；The best timeforstem segment colection suitable for in vitro induction culture was in summer;The MS solid medium supplemented with 1mg/L （20 6- Benzylaminopurine （6-BA） ， 0.05mg/L indoleacetic acid（IAA） 0.5mg/L （204号 gibberelin（GA3）was beneficial tothe germination adventitiousbuds.【Conclusion】The most suitablecultureconditions foradventitiousbudinduction grapefruitexplants werepreliminarilyscreenedout，therootstocksedculturemethodwas preliminarilydetermined.Thegrapefruit Seedlings Grafted bymicrobud grafting technologygrew wellInthefuture，the grafting survivalratecould be improvedbyimproving the grafting technology，soasturther enhancetheappicatio value.

Key words： Shui Jing Xiang pomelo； in vitro culture；hormone ratio；micro bud grafting

柑橘是重要的经济作物之一，国家统计局数据显示，2022年底我国柑橘种植面积已达2 995.81hm2 、年产量突破6000万t，占全球总产量的 1/3[1-2] 。（剩余4869字）