大肠埃希菌QD68株对碳青霉烯类耐药的分子机制

打开文本图片集

[中图分类号］R965 [文献标志码］A [文章编号] 2096-5532（2025）04-0609-04

doi：10.11712/jms.2096-5532.2025.61.131

[Abstract]ObjectiveTo investigatethe molecular mechanism of carbapenem resistance in Escherichiacoli strain QD68. MethodsTheVITEK2Compactsystemwasused toperforminitialidentificationandantimicrobialsusceptibilitytestingof strains，and Average Nucleotide Identity（ANI）wasusedforfinalidentificationof strains.NG-Test@CARBA 5asay was usedto detect carbapenemase.Ilumina MiSeq and Nanopore MinION sequencing platforms wereused toobtain the genomic sequecesof strains.Plasmidconjugationassyand S1nucleasedigestion pulsefieldgellectrophoresis wereused toanalye theplasmidsarried bythe strains.ResFinder4.1，PlasmidFinder2.0.1，andRASTwereusedforthebioinformaticsanalysis.ResultsThebioinfor matics analysis showed that E .coli strain QD68 carried one chromosome and four plasmids，which were named QD68-chr，pQD68- 1，PQD68-2，pQD68-NDM，and pQD68-IPM，respectively.This strain alsocariedtheblaNM-5andblaMP-8genes，whichwerelo cated on thepQD68-NDMplasmidand pQD68-IPM plasmid，respectively.Inadition，E.coli strain QD68couldtransferthe bla IMp-8gene to E.coli J53AziR through plasmid conjugation. ConclusionThe molecular mechanism underlying carbapenem resistance in E.coli strain QD68 may be attributed to the co-harboring of and blaIMP-8 genes. Therefore，it is necessary to monitor carbapenem-resistant Escherichia coli.

[Key words]Escherichia；carbapenemase；drug resistance，bacterial； bla NDM-5 ；bla IMP-8

抗菌药物耐药性给临床和公共卫生带来了重大挑战[1]。（剩余6632字）