利用SSR标记鉴定不结球白菜春福种子纯度

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中图分类号：S634.3 文献标志码：A 文章编号：1673-2871（2025）08-161-05

Hybrid purity identification of Brassica campestris ssp. chiensis variety Chunfu by SsR marker

SUN Zhongwei'，LU Chenchen²，XUYoulian，LI Manman³，GUO Tao²，GAO Qihui² （1.HenandtroleloentCoon;awrot Co.LD.，niicuralli Abstract：Currently，theassessmentofseedpurityinBrassicacampestrisprimarilyreliesonfieldidentificationetods. Thesemethods are notonly time-consuming and labor-intensive，butalso susceptible toenvironmental factors.To ensure thequalityofseedsentering themarketandto expeditethemarketpenetrationofChunfuseeds，thisstudydevelopedan eficientidentificationmethodfor Chunfuseeds bymolecular marker technology.TodevelopaneficientmethodforidentificationtheseedpurityofBrasicacampestris（syn.Brasicarapa）sp.chiensisvarietyChunfu，threeSSRprimers were selected from96SSRprimer pairs that exhibitedpolymorphism between the parental linesofChunfu.Among these，Rapa57demonstratedsuperior performance and deemedsuitable for purityidentification.TheRapa57 was selected to identify the seeds purity of Chunfu. Molecular detection matched field phenotypic identification with 1.9% deviation， the consistency was 98.1% .These results indicated that SSR marker can be applied to identify the purity of Brassica campestris. This methodapproach enhances identificationeficiency，exhibits excelentstabilityandaccuracy，alsoprovidesarobust scientific basis for seed production and quality control.

Keywords：Brassica campestris ssp.; Chunfu; Hybrid; Purity identification

不结球白菜[Brassicacampestris（syn.Brassicarapa）ssp.chiensis]，又称小白菜、胶菜、瓢儿菜、飘儿白、油菜，属于十字花科芸驀属芸驀种不结球白菜亚种。（剩余8299字）