萝卜种质资源抗根肿病鉴定及QTL-seq定位

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中图分类号：S631.1 文献标志码：A 文章编号：1673-2871（2025）08-037-08

Identification of clubroot resistance in radish germplasm resources and QTL-seq mapping

DANG Zengqing， QIAO Huifang （KaifengProductQualityInspectionandTestingCenter，Kaifeng475o4，Henan，China）

Abstract：Clubrootdisease，causedbyPlasmodiophorabraicaeWoronin，isagloballydistributedsoil-boredisease thatseriouslythreatens thesustainabledevelopmentof the Brassicavegetable production.Toclarifytheresistance levels ofradish germplasmresources and identify resistance loci，this studysystematicall evaluated the clubroot resistanceof 40radishvarieties trough integrated fieldnatural infectionandseedling-stageartificialinoculation methods.Segregating populations constructed from resistant and susceptible parents were subjected to QTL-seq-based mapping analysis.The resultsdemonstrated thatonelocalvarietyandfour hybridsexhibitedimmunity（I），onelocalvarietyand thre hybrids showed high resistance（HR），and threelocal varietiesshowed disease resistance（R）.QTL-seq analysis identified fouresistance-associatedQTLsonchromosomes1，2，and5，withthemajor-effectlocusrs2.1locatedonchromosome2.This research sucessullyscreens multiple elite resistance sources and maps clubroot resistance loci inradish，providing crucial scientific foundations for disease-resistant breeding and gene cloning.

Key Words： Radish; Clubroot disease; Identification; QTL-seq mapping; Disease-resistant breeding

萝卜（RaphanussativusL.）是我国重要的十字花科根菜类蔬菜，具有较高的营养价值和经济价值，常年种植面积约120万 hm2[1-2] 。（剩余9449字）