辣椒花药培养再生植株倍性鉴定方法的比较

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中图分类号：S641.3 文献标志码：A 文章编号：1673-2871（2025）08-077-06

Comparison of ploidy identification methods for regenerated plants from pepperantherculture

CHENG Zhifang， LI Li， SHI Yanyan， HAN Yanan， CHANG Xiaoke， YAO Qiuju （InstituteofVegetable，HenancdemyofAgriculturaliencesZhengzhouoo2Heania）

Abstract：Inorder toexploreanacurate，eficient，andapplicable ploidyidentification method forregenerated plants from pepperantherculture，inthisstudy，6Oregenerated plantlines from1O genotypesofpepperantherculture wereused as the test materials.The ploidy identification was conducted by adopting the plant fertilitydetection method and DNA flowcytometry，combined withthechromosomecounting methodofroot tipcellsquash.Theadvantagesanddisadvantagesof diferent methods werecompared.Theresults indicated thatthe accuracyrateof ploidy identification byDNA flow cytometry was 96.67% ，this method was rapid and convenient，but it had highcost and was prone to peak bias.The pollen vitality detection method had an accuracy rate of 100% in determining the odd-even ploidy of regenerated plants' chromosomes，but itcouldnotdistinguishthe specific ploidyofregeneratedplants.Thecombinationofpolenvitalitydetection method and DNA flowcytometry detection method could significantlyreduce the identificationcost，improve theaccuracyrateandeficiency.Thatis，forthelines thathavealreadyflowered，thepollenvitalitydetectionmethodcouldbeused to determine theodd-even ploidy of chromosomes，which was convenient for early chromosome doubling treatment; for thelines thatfloweredlater，theDNAflowcytometrydetectionmethodshouldbeused inthesedlingstage.Ifthere were difficult-to-determine situations suchaspeakbias，thepollnvitalitycouldbecombined todetermine the ploidyofregenerated plants.

KeyWords： Pepper;Ploidy identification; Flowcytometer; Pollen viability; Chromosome counting

辣椒（CapsicumannuumL. ，2n=2x=24 ），是世界上广泛种植的蔬菜作物之一，现有栽培品种多为一年生草本。（剩余6861字）