醋栗番茄抗灰霉病的基因定位和抗性生理研究

打开文本图片集

中图分类号：S641.2 文献标志码：A 文章编号：1673-2871（2025）06-026-09

Gene mapping and physiological study of resistance to Botrytis cinerea in gooseberry tomato

JIANG Jianing， XUE Dongqi， LOU Xueyuan， JIANG Liwei， GUO Xin （CollegeofHorticulture，HenanAgriculturalUniversity，Zhengzhou45o46，Henan，Cina）

Abstract：Graymold，causedbyBotrytiscinerea，posesserious threats toboth tomatoproductionandpost-harvestpreservation. This study utilized gooseberry tomato（LA2076），cultivated tomato（LA1246），and their F2 populations as experimental materials toanalyze thephenotypicand physiological differences between thesetwo typesof tomato varieties.By integrating the initial localization interval obtained through segregationpopulationgrouping analysis，22 InDelmarkers weredeveloped forfine mappingof tomatoresistance genesagainstgraymold.Theresults indicated thatat thesame time pointafterinvitro leaf inoculation with B .cinerea，the lesionareainLA2o76waslargerthanthatobserved inLA1246. TheMDAcontent exhibitedaninitialincreasefolowedbystabilization，withLA2O76displayingafasterrateofincrease thanLA1246.The activities of POD（peroxidase），CAT（catalase），and SOD（superoxide dismutase）allexhibited initial increasefollwedbydecrease，withLA2O76reaching higherpeak antioxidant enzymeactivitiesatearlier time points than LA1246. Genetic mapping revealed that the gene conferring resistance to gray mold was localized to a 4.29Mb interval flankedbyInDel-1.2andInDel-4.1onchromosome9，whichcontained38genes.Thisresearch providesatheoretical foundationfor guiding genetic breeding efforts aimedat enhancing tomato resistanceto graymold as wellas for furtherinvestigations into the genetic mappingand molecular mechanisms underlying this resistance.

Key Words： Tomato; Gray mold; Gene mapping; Resistance physiology

番茄是全球范围内大面积栽培的重要经济作物，果实具有较高的营养价值。（剩余9099字）