淹水胁迫下甜瓜幼苗的转录组测序及生物信息学分析

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中图分类号：S652 文献标志码：A 文章编号：1673-2871（2025）06-014-12

Transcriptome sequencing and bioinformatics analysis of melon seedlings under waterlogging stress

WANG Yanan， JIANG Yanfang，LIU Zefa，DANG Jiancheng， ZHANG Honglei， ZHANG Zixia （HunanUveityHumities，iencedhnologyoudiooHunina）

Abstract：Waterlogging isoneof themost widespreadand frequent natural disasters worldwide.Asacucurbit crop，melon exhibits relatively poor waterlogging tolerance.When melon cultivation areas experience prolongedrainfallor flooding，severeyieldloses orevencompletecropfailureoftenoccur.Inthisstudyawaterlogging tolerant melonhighgeneration M13-06-3，wasused as experimental material. Transcriptome sequecing and quantitative real-time PCR（qRT-PCR） were employed to analyze diferentially expressed genes（DEGs）inroot tissues.The results revealed that 9037 DEGs were screned in M13-06-3 under waterlogging stress including 4529 upregulated and 4508 downregulated genes. qRT-PCR validationconfirmed that the expression patternsof five genes，MELO3Co03917，MELO3Co20588，MELO3C008314，MELO3C025360，and MELO3C021658，were largely consistent wiht the transcriptomedata，suggesting their potentialrole inmelon waterlogging tolerance.Thesefindings provideafoundationforfurther explorationof waterlogging resistant genes in melon.

Key words：Melon;Waterlogging stress;Real-time fluorescence quantitativePCR;RNA high-throughput sequencing; Differentially expressed gene

葫芦科瓜类作物耐涝性较差，尤其是甜瓜，甜瓜属一年生草本葫芦科植物，喜温热气候，生长环境偏爱干燥排水良好的土壤，对涝湿环境耐受能力较弱，是我国重要经济作物。（剩余17114字）