乳腺癌患者外泌体miR-155的诊断价值及其与免疫炎症状态的关系

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DOI：10.16424/j.cnki.cn32-1807/r.2025.05.001

[中图分类号]R737.9 [文献标志码]A [文章编号] 1674-7887（2025）05-0411-05

Diagnostic value of exosomal miR-155 and its association with immune-inflammatory status in breast cancerpatients*

JIEJing1*，YAO Xiaomin²，ZHOUYuxiang²，YANGPengxiang²，YAOLei，ZHUYa** （Department of Clinical Laboratory，theFirstPeople'sHospitalofantong，Jiangsu226Ol4;KeyLaboratoryofNeuroregenerationofJiangsuProvinceand MinistryofEducation，Nantong University;DepartmentofAnesthsiologyDepartmentofPathology，theFirstPeople'sHospi tal of Nantong）

[Abstract]Objectie：ToinvestigatethexpressionlevelofexosomalmicroRNA-55（miR-155）intheperipheralbloodofbreast cancerpatients，itsassociationwithimmunefunctionandinflammatoryfactors，andtoevaluate itsdiagnosticvalue.Methods： Peripheralbloodsamples werecollctedfrom102breastcancerpatientsand51healthyfemales（control group）.Exosomal miR155and tumor markerCA153 levels were measured.Immunecell subsetsandserum inflammatorycytokineswereanalyzed. DiagnosticeficacyasassessedusingROCcurves.Inadition，Tcellsweretransfectedinvitrotoverifytheregulatorymehanism of miR-155.Results：Exosomal miR-155 was significantly elevated in breast cancer patients（ P< 0.05）and was closely associated with lymph node metastasis，distant metastasis，advanced stage，and triple-negative subtype（ Pe <0.01）.Patients exhibited a decreased CD4+/CD8 + ratio and natural killer（NK） cell activity，increased regulatory T cell（Treg） proportion，elevated IL-6，IL-10，and TNF- ∇⋅α∝ levels，and reduced IFN- γ levels（ P- <0.05）.miR-155waspositivelycorrelated with Treg，IL-6，IL-10， and TNF- α ，while negatively correlated with CD4+/CD8 + ratio，NK activity，and IFN- γ .ROC analysis showed that miR-155 hadanAUCof0.802forbreastcancer diagnosis，higherthan CA153（O.723）.Combined detectionofmiR-155and CA153 achievedanAUCofO.843with improvedsensitivityandspecificity.InvitroexperimentsindicatedthatmiR-155promoted IL-6secretionbyinhibitingsuppresorofcytokinesignaling1.Conclusion：ExosomalmiR-155ishighlyexpressedinbreast cancer.Itscombined detectionwithCA153 enhances diagnosticaccuracy，suggesting itspotentialasa liqud biopsybiomarker for early screening and personalized therapy.

[Keywords]breastcancer;exosomes;microRNA-155;immune-inflammatory profile;non-invasive diagnosis

乳腺癌是女性中最常见的恶性肿瘤类型，也是导致女性癌症死亡的首要因素之一[-2。（剩余6964字）