决明子醇提物对金黄色葡萄球菌的体外抑菌作用研究

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文章编号：2096-8108（2026）03-0044-04 中图分类号：R285.5；Q939.91 文献标识码：A

In Vitro Antibacterial Effect of Alcohol Extract from Cassia Seed against Staphylococcus Aureus

CHEN Yanfei

（Taiyuan Normal University， Taiyuan Shanxi ， China）

Abstract： 【Objective】To investigate the in vitro antibacterial activity of alcohol extract from cassia seed. 【Methods】Alcohol extracts of Cassia obtusifolia were prepared at concentrations of 0.25， 0.50， 0.75， 1.00， 1.50， and 2.00 g/mL. The inhibition zone diameters against Staphylococcus aureus were determined using the filter paper method and the Oxford cup method， with sterile water as a blank control. 【Results】The alcohol extract of Cassia obtusifolia exhibited significant antibacterial activity against Staphylococcus aureus in a concentration-dependent manner. The strongest antibacterial effect was observed at the concentration of 2.00 g/mL， with inhibition zone diameters of 25.6 mm （filter paper disc method） and 28.4 mm （Oxford cup method）. The weakest effect was observed at 0.25 g/mL， with inhibition zone diameters of 13.6 mm and 13.4 mm， respectively. The Oxford cup method showed superior antibacterial effects compared to the filter paper method. 【Conclusion】The alcohol extract of Cassia obtusifolia possesses good in vitro antibacterial activity against Staphylococcus aureus， and the antibacterial activity is positively correlated with the extract concentration. These findings provide a preliminary scientific basis for the integrated utilization of Cassia obtusifolia in ecological fruit tree cultivation and fruit food safety.

Key words： semen cassiae alcohol extract; staphylococcus aureus; diameter of inhibition zone; filter paper method; oxford cup method

近年来，利用果园间作具有抑菌活性的中草药植物，以降低土传病害和果实表面病原菌的侵染，成为果树生态栽培和果实采后绿色保鲜的研究热点之一。（剩余4491字）