宏基因组下一代测序在老年肺部感染中的应用与价值探讨

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[Abstract]ObjectiveTo explore thedetection efect metagenomic next-generationsequencing（mNGS）technologyon elderlypulmonaryinfections.MethodsAtotal289elderlypatientsagedover6OyearswithclinicallysuspectedpulmonaryinfectionsadmitedtotheRegionfromJuly2O21toDecember2O23wereselectedasstudy subjects.AllpatitsuderentmNGohosopitrchalaspiatesandconvtioalmicrobilgicaltestsedagoicpe formancemNGandconventionalmicrobiologicaltestswascomparedusinglinicaldiagnosticresultsasthegoldtandardResults Elderlypulmonaryinfectioswerpredominantlyolcrobial，accoutigfor4.5%（117/89）.ediagosticaccuracyadsitivity mNGS were 78.5% and 89.2% respectively，surpassing those conventional microbiological test （ 37.4% diagnostic accuracy， 28.8% sensitivity）.Forthe majoritypathogens，thedetectionrate mNGSwashigherthanthatconventionalmicrobiological test.The polymicrobial infectionrate inimmunocompromisedpatientswas73.1%（38/52）.Thepathogenspectrumwasalsoobserved tovaryamongdiferentpopulations.Thepositivepredictivevaluewasfoundtodependonthetypepathogenandthepatient'smmune status.ConclusionInelderlypatientswithpulmonaryinfections，polymicrobialinfectionsarethemostcommon.mNGScangreatly improvetheaccuracyanddetectionatepathogensinelderlypatientswithpulmonaryinfections.Inaddition，wheninterpreting mNGSresults，thecomorbiditiesandtypespathogensshouldbeconsidered.mNGShasahighclinicalapplicationvalueinhediagnosis pulmonary infections in elderly patients.

[Key words] Metagenomic next-generation sequencing；Tracheal aspiration；Pulmonary infection； Pathogen detection；Comorbidity

肺部感染是威胁老年人健康和生命的关键疾病之一。（剩余12887字）