正交试验法优选小儿清热消积口服液水提工艺

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【中图分类号】R284.2 【文献标志码】A 【文章编号】1007-8517（2025）16-0049-05

DOI： 10.3969/j .issn.1007-8517. 2025.16. zgmzmjyyzz202516011

Optimization of Water Extraction Process for Xiaoer Qingre Xiaoji Oral Liquid by Orthogonal Test

LI Dongdong HOU Baozhen*ZHANG Dandan XING Yunyun Jining Hospital of Xiyuan Hospital （Jining Hospital of TCM），Jining 272OOO，China

Abstract：ObjectiveTooptimizethewaterextractiontechnologyof XiaoerQingreXiaoji OralLiquid.Methods Usingextraction times，extractiontimeandwateramountasfactors.Takingthecomprehensivescoreofthedryexractyieldandcontentofberberine hydrochlorideinthextractastheevaluationindex，orthogonaltestwasemploydtooptimizetheextractiontechnologyofXioeringre Xiaojioralliquid.ResultsThebestseparationconditionsareasfolows：themobilephasewasacetonitrileandO.O5mol/LKH2PO4 solution （adding sodium dodecyl sulfate 0.72 z per 100 mL and then adjusting pH to 3.0 byphosphoric acid）（50：50），the detected wavelength was 265 nm and the column temperature was 30Y .The concentration of berberine hydrochloride showed a good linearity in the ranges of 3.10-12.40 μg/mL （ r=1.000 0 ）．Additionally，the average recovery was 101. 18% ，RSD was 1. 83 % （ n=9 ）·Theoptimalextractionprocess wasadding10foldsof water，extractingtwice，extracting for1.5heachtime.ConclusionTheoptimizedprocessisfeasibleandstable，whichcanprovidedatasupportforthestandardizedproductioofXiaoerQingreXiaojioraliquid.

Keywords：Berberine Hydrochloride；Orthogonal Test；Water Extraction Technology

小儿清热消积口服液是我院儿科多名专家在长期的临床实践中，以中医药理论为指导，总结出来的治疗小儿食积内热证的临床经验方。（剩余5590字）