经鼻枸缘酸铁铵处理对小鼠嗅球突触囊泡周期 通路的影响

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[关键词]铁；嗅球；转录组测序；突触;囊泡周期通路；PC12细胞[中图分类号］R338.2 ［文献标志码］A [文章编号] 2096-5532（2025）06-0796-04doi：10.11712/jms.2096-5532.2025.61.177 [开放科学（资源服务）标识码（OSID）

[网络出版]https：//link.cnki.net/urlid/37.1517.R.20251218.0952.001; 2025-12-1907：27：01

Impactof transnasaladministrationoffericammoniumcitrateonthesynapticvesiclecyclepathwayintheolfactorybulbof mice WANG Xiaoting，WANG Dongxia，XIE Junxia，SONG Ning（Department of Physiology and Pathophysiology，Scholof Basic Medicine，Qingdao Medical College of Qingdao University，Qingdao ，China）

[Abstract]ObjectiveTo investigate the impactof transnasal administrationofferic ammonium citrate（FAC）on the sy naptic vesiclecycle pathway inolfactory bulb（OB）tissue，and toass theconsistencyof thisefect in PC12cells.Methods Mice were dividedintoFACtreatment groupandcontrolgroup.OBtissue wascolected，andRNA sequencing（RNA-seq）was performedtoidentifydiferentiallyexpressedgenesassociated withthesynapticvesiclecyclepathway；qPCRwasusedtovalidatethe changes in the expression of the genes such as Slc17a8 ，Slc6a12，and Uncl3c ，which was verified in PC12 cells. Results RNAseqshowedthatFACtreatment significantlydownregulatedtheexpresionof the genesasociated withthesynaptic vesiclecycle pathwayin OB tissue.The results of qPCR validation further confirmed significant downregulation of the Slcl7a8 ，Slc6a12，and Unc13c genes in both OB tissue τ=3.184-4.285 ， P<0.05 ）andPC12cells Δt=4.540-13.350 ， P<0.05 ），which was consistent withthe resultsofRNA-seq.ConclusionIrontreatmentcandownregulatetheexpresionofthegenes associated withthe synaptic vesiclecyclepathwayinOBtisseandPC12cell，suggestingthatironmay impair thenormalfunctionofthesyapticvesicle cycle pathway.

[Key words]iron；olfactory bulb；RNA-seq；synapses；synaptic vesicle cycle；PC12 cells

铁在大脑中参与氧运输、神经递质合成、髓鞘形成、线粒体能量代谢、细胞分裂等过程[1-2]。（剩余8405字）