外侧苍白球注射 FeCl₃ 对小鼠运动行为的影响

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[关键词] 铁蛋白质类；苍白球；运动活动；帕金森病；小鼠[中图分类号］R338.2 [文献标志码］A [文章编号] 2096-5532（2025）06-0791-05doi：10.11712/jms.2096-5532.2025.61.176 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20251218.1024.005；

2025-12-19 11：37：53

Effect of FeCl3 injection into the external segment of the globus palidus on motor behavior in miceLIU Baochen，WANG Dong.cia， XIEJunxia，SONG Ning（DepartmentofPhysiologyand Pathophysiology，Schoolof Basic Medicine，Qingdao Medical Coege ofQingdaoUniversity，Qingdao 266071，China）

[Abstract] Objective To investigate the changes in motor behavior of mice after FeCl3 injection into the external segment of the globus pallidus（GPe）.MethodsMice were divided into FeCl3 group and control group，with 8 mice in each group. The mice in the FeCl3 group were given stereotactic injection of 0.15μg of FeCl3 into the bilateral GPe，and those in the control group were given njectionofanequal volumeofsodiumcitratesolutionAfter modeling，behaviraltestswereconductedatweks 3and6，and theCatWalk XTsystem wasusedtoevaluatemotorbehavior.Afterthebehavioraltestat week6，Perl's-DABstaining wasused to measure the number of iron-positive cells in the GPe ，and Western blotting was used to measure the expression level of heavy-chain ferritin in the GPe.ResultsThe FeCl3 group had significant increases in the number of iron-positive cells in the GPe t=3.887 ， P<0.01 ）andthelevel ofheavy-chain ferritin （t=5.011，P<0.001） ，suggesting that stereotactic injection of FeCl3 could cause iron deposition in the GPe. The gait analysis showed that the FeCl3 group had a significant reduction in the time to cross the testingarea and a significant increase in average speed （week 3： t=2.771，3.392，P<0.05 ；week 6： t=2.526 ， 2.336，P<0.05 ，as well as significantincreasesinthespeedsoftheleftforelimb，thelefthindimb，therightforelimb，andtheright hindlimb（wek 3： t= 3.270-3.725，P<0.05 ;week 6 ： t=2.419-3.249，P<0.05） .ConclusionStereotactic injection of FeCl3 into the GPe of mice can cause iron deposition in the GPe and enhance the motor ability of mice.

[Key words]ferritins；globus pallidus；motor activity；Parkinson disease；mice

苍白球（GP）由外侧GP（GPe）和内侧GP（GPi）构成，是基底神经节的重要组成部分，是运动调控的关键脑区，并与诸多神经系统疾病有关[1-2]。（剩余10704字）