双链DNA胞苷脱氨酶检测DNA甲基化

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中图分类号：R34 文献标志码：A

Double-stranded DNA Cytosine Deaminase Detects DNA Methylation

TANG Yue，LI Xin-min（Institute of Translational Medicine，Qingdao University，Qingdao 266o21，China）

Abstract：A highly sensitive double stranded DNA cytosine deaminase DddA coupling methylation-specific PCR method was developed for detecting DNA methylation， avoiding DNA sample degradation in bisulfite conversion coupled Methylation-Specific PCR. In DddA conversion-based methylation specific real-time quantitative PCR，the DddA deaminated the DNA samples firstly，then performed the real-time PCR with methylation specific primers. According to standard curve of the target genes，the methylation level of the DNA sample could be evaluated. The results showed that the DddA can discriminate the methylate with unmethylated DNA well. And DddA-qMSP can effectively detect the methylation status of colorectal cancer marker genes SEPT9 and SDC2.

Keywords： double-stranded DNA; double-stranded DNA cytosine deaminase; cytosine methylation; epigenetic inheritance; genetic testing

DNA甲基化（DNA Methylation）是常见的表观遗传修饰之一，不发生DNA序列改变，但影响基因表达[1]和基因组稳定性[2]。（剩余11564字）