肠道高黏附性噬菌体对小鼠沙门氏菌感染的预防作用

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Abstract：In order to study the preventive effct of adherent bacteriophages against Salmonella infection in mice，a gut-adherentbacteriophagevB_SpuM_PA1-27wasisolated.ByestablishingamurineSalmonellinfectionmodel，thisstudy investigated thepreventive effect of vB_SpuM - PA1-27 against Salmonella infection through four experimental groups：blank control，bacteriophagecontrol，Salmonella-infectedgroup，andbacteriophage preventiongroup.Thersultsdemostrated that inthe bacteriophage prevention group，vB_SpuM_PA1-27wasdetectable throughoutallintestinal segments，withhe highest abundance in colon. Concurrently，Salmonella loads in fecal samples were significantly reduced （ P<0.05 ），and no mortalityoccurredinmice.ComparedwiththeSalmonell-infected group，phagepretreatmentsignificantlyreduced the spleen index （ P<0.05 ）and mRNA expression levels of TNF- α and μ Iβ in colon （ P<0.05 ），and significantly increased

jejunal villuslength（ P <0.05），colon length（ P<0.05 ）， and MUC2 mRNA expression（ P<0.05 ）．The results indicatedthatvB_SpuM_PA1-27couldmitigateSalmonella -induced pathogenic impacts in mice，preserve intestinal healthand reduce mortality. These findings can provide an important data reference forthe application ofadherent bacteriophages in the preventionof bacterial infection in animals.

Keywords：adherent bacteriophages；intestinal health；infection model；Salmonel；mice

沙门氏菌是养殖场主要的细菌感染病原菌，导致种鸡和肉鸡疾病以及食品安全问题。（剩余10699字）