春尺蠖保幼激素酯酶基因AcinJHE生物信息学与表达分析

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关键词：春尺蠖；保幼激素酯酶基因（JHE）；低温胁迫；饥饿胁迫中图分类号： S888.72AA+1 文献标识码：A 文章编号： 1000-4440（2025）09-1721-08

Abstract：To investigate therole of juvenile hormone esterase（JHE）in the growth，development，and environmentaladaptationof Apocheimacinerarius，thisstudyscreenedand identifieda JHE genebased on the transcriptomic data of A.cinerarius viahomologous alignment，and named itAcinJHE.The CDSsequenceof this gene was1755bpin length， encodinga protein composed of 59O amino acids.The predicted relative molecular weight of the protein was 65580，with anisoelectric point of 5.70.It contained a signalpeptideof25aainlength，lackedtransmembrane structures，and itscarboxylesterase conserved domainwas locatedat39-579aa.Phylogeneticanalysisresultsshowed that AcinJHE had the closest genetic relationship with the JHEof Operophtera brumata，clustering on the same

branch.Fromthelarvalstagetothenon-diapausepupal stage，therelativeexpressionlevelofAcinJHEincreasedsignificantly （ P<0.05 ）.Fromthediapause pupal stage to the diapause-terminated pupal stage，the relative expresson level of AcinJHE in A .cinerarius decreased significantly（ P<0.05 ）.Therelative expressionlevel of AcinJHE was the highest in the headsof male adults.The AcinJHE gene responded to low-temperatureand starvation stresss.Afterthreehours of low-temperature treatment at 4 C ，the relative expresson level of the AcinJHE gene in the 3rd-instar larvae of A.cinerarius reached thepeak.After72 hours of starvation treatment，the relative expressionlevel of the AcinJHE genein the4th-instarlarvae of A.cinerariuswas significantly higher than that in the control group （ P<0.05 ）.This study elucidates the molecular mechanismoftheAcinJHEgene inregulating thegrowth，development，andstressadaptationofA.cinerarius，therebyprovidinga theoretical basis for its control.

Key words：Apocheima cinerarius；juvenile hormone esterase（JHE）；low temperature stress；starvation stress

春尺蠖（Apocheimacinerarius）属鳞翅目尺蛾科（Geometridae），是新疆、内蒙古、河北等北方地区荒漠草原及人工林区的主要杂食性害虫[1]。（剩余11734字）