高糖枇杷果实发育后期淀粉和蔗糖代谢的转录组分析

打开文本图片集

中图分类号：S667.3 文献标识码：A 文章编号：1000-4440（2025）09-1833-1

Abstract：In this study，a systematic transcriptome analysis was conducted on loquat fruits at five stages，namely the150thdayafterflowering（Ej-FS1），the160thday（Ej-FS2），the170thday（Ej-FS3），the180thday（Ej-FS4），and the190th day（Ej-FS5）.Atotalof 17564 diffrentiallyexpressed genes were screeed fromthecomparison groupsof EjFS1andEj-FS2，Ej-FS2andEj-FS3，Ej-FS3andEj-FS4，andEj-FS4andEj-FS5Therewere8584diferentiallyexpressed genes inthecomparison groupofEj-S3andEj-FS4，among which243diffrentiall expressed genes wereenriched inthestarch andsucrosemetabolismpathway.Thesediferentiallyexpressed genesregulated25enzymessuch ascytosolic neutral invertase， sucrose-6-phosphatase，sucrose phosphate synthase，sucrose synthase，fructokinase，and glucose-6-phosphate isomerase in the starch and sucrose metabolism pathway.The expression patterns of the differentially expressed genes detected by qRT-PCR were consistent with the transcriptome data，indicating that the transcriptome data obtained through high-throughput sequencing in this study are reliable.The results of this study reveal the molecular mechanism of sugar accumulation in loquat fruitsand provide important candidate genesand a theoretical basis formolecularbreeding ofloquat.

Key words：loquat；sugar accumulation；transcriptome；differentially expressed genes

糖类物质的组成、含量及动态积累是决定果实风味品质的关键因素1，在果实中的糖代谢包括淀粉的积累与降解、蔗糖的积累与降解以及己糖的积累和再利用3个阶段2，涉及蔗糖代谢途径、山梨醇代谢途径、己糖代谢途径和淀粉代谢途径4大代谢途径[1]糖分积累与源库关系存在双向调控机制：糖分积累既受源库关系的动态调节，又通过反馈机制影响源库分配[3]。（剩余12453字）