江苏省南通地区虾源弧菌的分子流行病学分析

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中图分类号： S945.4 文献标识码：A 文章编号：1000-4440（2025）09-1791-12

Abstract：Toelucidatethe molecular epidemiological characteristicsof shrimp-derivedVibrios inNantong area，Vibrio strains isolatedfrom 56Odiseased shrimpsamplescolected from large-scale shrimpfarms in Nantong area were dentified，andtheantimicrobial susceptibilityoftheisolateswas testedbymicro-brothdilution method.Basedonthewholegenomesequencingtechnology，thedrug resistance genesandvirulence genesof theisolates wereanalyzed，andtheepidemi

ological characteristicsofshrimp-derivedVibriosin Nantong area were analyzed by multilocus sequence typing （MLST）and phylogenetictree.The resultsshowedthat 119strains ofVibrio were isolated from 56O diseased shrimp samples，with a detection rate of 21.25% . Among them，Vibrio alginolyticus was the dominant subtype，accounting for 73.95 % .Theresistance ratesof the isolatesto ampicillin，cephalexin and doxycyclinewere 73.11% ，

58.82% and 45.38% ，respectively.A total of 47 drug resistance genes were detected inthe isolates，among which （204号 tet（34） ， tet（35） and blaampC had higher detection rates of 97.48% ， 88.24% and 61.34% ，respectively. The isolates carried an average of 33 virulence genes，and the numbers of isolates carrying the key cytotoxin-encoding genes hlyA and rtxA were oneand seven，respectively.The STtypes of the isolates were diverse，and ST388 was arelatively popular STtype.The isolates of V. alginolyticus were divided into one evolutionary branch，and the genetic evolution relationship between the strains from diferent sources was close. The isolate 85 and isolate 4 were highly homologous to the V. alginolyticus strains from humanclinical sources.Theresultsofthisstudycanprovideascientificbasis fortheefective preventionandcontrolof Vibrio infections in aquaculture in Nantong area.

Key words： Vibrio；shrimp；drug resistance genes；virulence genes；evolutionary typing

弧菌为革兰氏阴性菌，广泛分布于海洋、河海交汇处及鱼虾贝类等水生动物体内。（剩余11641字）