蚕豆NAC转录因子家族鉴定分析及抗叶斑病基因筛选

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中图分类号：S435.23 文献标识码：A 文章编号： 1000-4440（2025）09-1683-13

Abstract：The NAC transcription factors play important roles in plant growthand development as wellasin responses toabioticandbioticstresses.ToclarifythefunctionsofNACtranscriptionfactors in ViciafabaL.，inthis study， bioinformatic methods were used to identify the members of the NAC family genes from the Vicia faba genome database. The genestructures，cis-actingelements in promoters，chromosomal distributions，andthe physicochemical propertiesofthe proteinsencodedbythesegeneswereanalyzed.ByintegratingthetranscriptomedataofViciafabainfectedbyAlternariaalternata（thepathogen causingleaf spotin Viciafaba），candidategenesrelated todiseaseresistance werescreened.Theresults showedthatthere were77 membersintheNACtranscriptionfactorgene familyof Vicia faba，which wereunevenly distributed on six chromosomes.Theencoded proteinsranged insizefrom133aa to 665aa.The VfNAC proteinswere

dividedinto 16subfamilies，allofwhich contained the conserved NAM domain and werelocalized to the nucleus. The promoters of VfNAC genes contained various cis -acting elements such as light-responsive elements，hormone-responsive elements，and drought-responsive elements.The transcriptome sequencing results showed that 33 VfNAC genesweredifferentiallyexpressed in Vicia faba infected byAlternariaalternata，and four genesexhibiting significant differential expression were selected as keycandidate

disease-resistancegenes forfurtherstudy.Thisstudyprovidesareferenceforthesubsequentanalysisof thebiological functions of NAC transcription factors in Vicia fabaand theresearch on the molecular mechanisms of disease resistance.

Key Words：Vicia faba L.；NAC transcription factor；Alternaria alternata；disease resistance genes

病原菌、虫害、草害等生物胁迫严重影响作物生长发育，导致作物产量和品质下降。（剩余14418字）