基于HPLC指纹图谱和一测多评法的化瘀止痛贴膏质量评价

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[关键词]化瘀止痛贴膏；质量评价；指纹图谱；一测多评；相似度评价[中图分类号]R284.1 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.12.009

Quality evaluation of Huayu Zhitong Plaster based on HPLC fingerprint and QAMS method

LIU Xiaohui 1，2 ，PENG Jiangli'，FENG Yanting²， DENG Meilingl²，QIAN Min I，2 WANG Shi3*， PENG Qiuxian12*

1.SchoolofPharmacy，Huncm UniversityfChineseMedicine，Changsha，Hunan410208，China;2.KeyResearchLaboratoryof Germplasm Resources and StandardizedPlanting of Genuine RegionalMedicinal Materials producedin Hunan Province， Changsha，Hunan41O2O8，China;3.PharmacyDepartment，theSecond HospitalofHunan UniversityofChineseMedicine， Changsha，Hunan 410005， China

[Abstract]ObjectiveToestablishtheHPLCfingerprintof Huayu ZhitongPlasterandtodeterminethecontentof itsten componentsusingthquantitativeanalysisofmulti-componentsbysingle-marker（QAMS）method，soastoprovideareferecefor quality assessment. Methods The HPLC fingerprint was developed'using an Agilent HC-C 18 column with a mobile phase of acetonitrile- .1% formicacid 0.1% triethylamine under gradient elution conditions.The column temperature was maintained at 30 C and the flow rate was set at 1mL⋅min-1 . Similarity evaluation was performed on the fingerprint， and chemometric methods were applied forchemicalpattrnrecognition.Thecontentofthecomponentswerecalculatedusingrelativecorectionfactors（RCFs）. TheresultsobtainedbytheQAMSmethodwerecomparedwiththosedeterminedbytheexternalstandardmethod，andaditional RCFswerecalculatedacordinglyforcontentdetermination.ResultsAtotaloftencommonpeakswereidentifiedinthefingerprints ofthe14batches，withsimilarityevaluationsexceedingO.9.Usingbaicalinastheinternalreferencesubstance，thecoection factorsforateinroxafloreloldenineirosde，gne，e，bie physcionweredeterminedas09229，1487，1.1716，537，11135，03168，0006，0.3894，and0.5944，respectively.ere wasnosignificantdiferencebetweentheresultscalculatedbytheQAMSmethodandthosecalculatedbytheexternalstandard method.Conclusion Theestablished HPLC fingerprintcombinedwith theQAMSmethoddemonstratesacuracyandreliability thereby providing ascientific foundation for thequalitycontrolandcomprehensive evaluationof Huayu Zhitong Plaster.

[Keywords]HuayuZhitongPlaster;qualityevaluation；HPLC fingerprint;quantitativeanalysisofmulti-componentsby single-marker;similarityevaluation

急性软组织损伤（acutesofttissue injury，ASTI）是常见的运动损伤，多由扭伤、挫伤及跌打导致，可引起肌肉、韧带、筋膜、肌腱、滑膜及周围神经、血管的损伤。（剩余8823字）