百合知母汤通过改善线粒体功能与自噬保护秀丽隐杆线虫多巴胺能神经元的机制研究

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[关键词］百合知母汤；秀丽隐杆线虫；氧化应激；线粒体功能；多巴胺能神经元 [中图分类号]R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.12.005

Mechanism of Baihe Zhimu Decoction in protecting dopaminergic neurons in Caenorhabditis elegans via enhancement of mitochondrial function and autophagy

CHEN Sangni， WU Mei， HE Wei， MENG Pan*，LI Dandan* Hunan University ofChinese Medicine，Changsha，Hunan4lO2O8，China [Abstract]Objective To investigate theprotectiveefectsof Baihe Zhimu Decoction （BHZMD）on dopaminergic neurons （DNs） in Caenorhabditis elegans （C. elegans）. Methods Synchronized C* elegans worms were randomly divided into blank control group， model group [exposed to 400μmol⋅L-1 rotenone （ROT）]， BHZMD group （treated with 6.7 mg·mL- BHZMD solution+400 μmol ·L- ROT）， and fluoxetine group （treated with 100μmol⋅L-1 fluoxetine solution+4OO μmol·L- ROT）. The optimal concentration of ROT was determinedbasedonthefluorescenceintensityofgreefluorescentprotein （GFP）andthedegreeofDNdeformityof theworms.The optimal concentrationof BHZMDwasselectedbyassessngwormheadthrash frequency.Dopaminergicneuronaldamagewas evaluatedinthewormswithGFP-labeledDNs（BZ55）.Mitochondrial functionwasanalyzedusing wormsexpresingfluorescent markersforelectrontransportchain （ETC）complexes（CB7272）tomonitorETCrecovery.Mitochondrialmembranepotentialwas measuredusingafluorescenceprobe.Survivalanalysiswasperformedinajuglone-inducedoxidativestressmodel.Intracelular reactiveoxygen species （ROS）levels were measuredusingtheDCFH-DAprobe.Theexpresionof pink-1 was examined inautophagyrelatedgene-labeled worms （RB2547）viaimmunofluorescence.Results TheoptimalmodelingconcentrationsofROTandBHZMD were determined to be 4 .00μmol⋅L-1 and 6.7mg⋅mL-1 ，respectively. Compared with the blank control group， the model group exhibited significantly decreased GFP fluorescence signals of DNs（ P< 0.01） and significantly increased degree of DN deformity（P<0.01）. Furthermore，the GFP intensity of the mitochondrial ETC complexes was significantly reduced （ P <0.01），along with decreased ATP levels （P <0.01）， elevated ATPlevels P <0.01）， improved mitochondrial membrane potential （ P <0.01）， higher survival rates （ P <0.01），decreased ROS levels （PO.O1），and intensified pink-1 fluorescence signals （ P

[Keywords]Baihe Zhiu Decoction;Caenorhabditiselegans；oxidativestres;mitochondrial function;dopaminergicneuron

多巴胺能神经元（dopaminergic neuron，DN）是中枢神经系统的关键部分，其损伤与神经系统疾病的发生发展密切相关。（剩余12829字）