基于网络药理学及分子对接技术探讨杞菊地黄丸治疗干眼症的作用机制

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[Abstract]ObjectiveToexploretheactiveingredientsand mechanismofactionof Qiju Dihuang Pilsinthetreatmentof dry eyebynetworkpharmacologyandmoleculardocking.MethodsTheactiveingredientsofQijuDihuangPilswereobtainedfromTCMSPand PharmacopoeiaofthePeople’sRepublicofChina，andtheirtargetswereconvertedintostandardgenenamesthroughtheUniProtplatfo. DiseasetargetserescrenedfromGeneCards，OMMandDisGeNETdatabases.TheCytoscape3.9.1softwareasusedtoconstructandanalyzetheompoundnetworktoobtaintemainactiveingredientsofthedrugProteininteractionsetwengenesintersectingwithQijuDihuangPillsnddryeereaalydoeGplafondtenmporedintothesoftaretoeenforyargetseesetio geneswereanalyzedforGOfunctionandKEGGpathwayenrichmentusingtheDAVIDdatabase，andfinaly，moleculardockingvalidation wascompletedbyAutoock4.2.6softare.Results149activeingredintsofQijuDiuangPilsand2O14dryeyeargetsweresreeed， withatotafil nin，stigmasterol，soetinndhargesicdedroteinaseB（AK）oosisctor），rote （TP53），nterleukin6（6），nteleukinbeta（B），strognreceptor1（ESR1）Keypathwaysicudedtedvanedglatiodproducts（AGE）-reeptorforadvancedglycationend-products（RAGE）signalingpathwayindiabeticcomplications，phospoiositide3- kinase（PI3K）-AKTsignalingpathwaymitogen-activatedproteinkinase（MAPK）signalingpathway，etc.ConclusionQijuDiuang PillsmaytratdyeytouhectiofqueceionyargessuchasTF5，6dsoItaexetattoryandinhibitapoptosisbyregulating theAGE-RAGEsignalingpathway，PI3K-AKTsignalingpathwayandMAPKsignalingpathway.

徐晨，等，基于网络药理学及分子对接技术探讨杞菊地黄丸治疗干眼症的作用机制

干眼症作为一种常见的多因素慢性眼表疾病，其主要病理特性是不稳定泪膜引起的不适甚至视力障碍，同时伴有眼表炎症、损伤及神经感觉异常[1]。（剩余14096字）