基于SIRT1-NF-kB/PGC-1α-BDNF信号通路探讨跑台运动和饮食控制对ApoE-/-小鼠模型认知功能的影响

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[关键词] 运动；饮食控制；高脂饮食；SIRT1-NF- κκB ；PGC- ∇⋅1α∝ -BDNF；认知功能doi：10.3969/j. issn.1674-7593.2025.03.007

[Abstract]ObjectiveTo investigate the efectand mechanismof treadmillexerciseand diet control oncognitive dysfunction in apolipoprotein E knockout （ApoE -/. -）mice model.Methods Forty ApoE -/. -male mice fed a high-fat diet for8 weeks were randomly and equally divided into a high-fat diet exercise （ HFD+TR ）group，a high-fat diet rest（HFD + SED）group，a diet-controlled exercise （DC +ΔTR ）group，and a diet-controlled rest （DC + SED）group，with 10 mice in each group. HFD + TR group and HFD + SED group continued to be fed with high-fat diet for 1O weeks，DC + TR group and DC + SED group switched to standard diet for10 weeks，and the food intake was controlled to 70% of the normal intake，while treadmill exercise intervention was performed in HFD + TR group and DC +ΔTR group.Morris water maze test was used to evaluate the cognitive function of mice，enzyme-linked immunosorbent assay（ELISA） was used to detect the serum levels of tumor necrosis factor- α （TNF- ∇⋅α∝ ）and interleukin- 1β （IL-1β），and real-timePCR（RT-qPCR）wasusedtodetect theexpresionlevelsofsilentinformationregulator1（SIRT1），nucleartranscription factor NF-kB（NF-kB），peroxisome proliferation receptor gamma coactivator α （PGC-1α），and brain-derived neurotrophic factor （BDNF）inthehipocampus.ResultsTheresultsof watermaze testshowedthatdietcontrolandtreadmillexercisecouldimprove spatiallearningandmemoryabilityofmice.TheresultsofELISAandRT-qPCRshowedthatdietcontrolandtreadmillexercisecould decrease thelevels of inflammatory cytokines TNF- α and IL- 1β in the serum of mice，and also decrease the expression levels of NF- κB （2 mRNA in the hippocampus of mice，but increase the expression levels of SIRT1，PGC- l∝ ，and BDNF mRNA in the hippocampus of mice.ConclusionTreadmillexerciseanddietcontrolcanreducetheinflammatoryresponseinthehippocampusofmicebymediating the SIRT1-NF-KB/PGC- 1α -BDNF signaling pathway，thereby improving cognitive function in mice.

[Key words] Exercise；Dietcontrol；High-fat diet；SIRT1-NF- ∇⋅κB ；PGC- 1α -BDNF；Cognitive function

认知功能障碍是一种常见的神经退行性疾病，其特点是记忆、语言、执行、计算和理解判断等认知功能中的一项或多项受损[1]。（剩余15663字）