猫栉首蚤携带立克次体、柯克斯体和巴尔通体的 分子检测与遗传演化分析

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中图分类号：S852.6；S852.745 文献标志码：A 文章编号：0366-6964（2025）09-4646-11

Molecular Detection and Genetic Evolutionary Analysis of Rickettsia，Coxiella ， and Bartonella Carried by the Cat Flea Ctenocephalides felis

FU Yitian1，WU Yali1，PENG Yanyan1， SU Si2 ， LIU Guohua1 *（1. College of Veterinary Medicine， Hunan Agricultural University， Changsha 41ol28， China ;2. Department of Pathology，Yueyang Central Hospital， Yueyang 4l40oo，China）

Abstract： The infection status of Rickettsia， Coxiella，and Bartonella in cat fleas Ctenocephalides felis collected from selected areas of China was investigated in this study. From January， 2019 to July，2023， 294 fleas were collected from the body surface of pet dogs and cats across multiple Chinese regions. After preliminary morphological identification，the ITS1 sequences of fleas，gltA and ompA gene sequences of Rickettsia ， IS1111 sequences of Coxiella ，and gltA and ropB genes sequences of Bartonella were amplified with PCR. The positive products were sequenced and then subjected to sequence alignment and phylogenetic analysis. The results showed that all of 294 collected specimens were identified as cat flea C . felis. Results of molecular detection and genetic evolutionary analysis demonstrated that R .felis，C.burnetii and Bartonella were found in cat flea C . felis，with prevalence of 13.3% （39/294）， 1.7% （5/294）and 25.9% （76/294），respectively. Among the infections of Bartonella， B . henselae was detected with a prevalence of 0.3% （1/294），B. koehlerae was 0.3% （1/294），B. clarridgeiae was 0.7% （2/ 294），and Bartonella spp. were 24.5% （72/294）. In addition，co-infections involving R . felis and Bartonella spp. were identified. The cat flea C . felis was identified as the predominant flea species infesting pet cats and dogs in China，indicating potential of C . felis as a vector for multiple zoonotic agents. It was proven for the first time that cat flea C . felis carried C . burnetii. Rickettsia and Bartonella carried by C . felis in China showed significant geographical divergence and genetic diversity.

Keywords： Ctenocephalides felis ； pet dog and cat; Rickettsia ； Coxiella ； Bartonella∗ Corresponding author： LIU Guohua，E-mail：liuguohua5202oo8@163.com

蚤目昆虫是一类小型，无翅，分布广泛的嗜血性体外寄生虫，因多数善于跳跃，故又称跳蚤。（剩余17686字）