滩羊超数排卵处理后血浆类固醇激素对超排效果的影响

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中图分类号：S826.3 文献标志码：A 文章编号：0366-6964（2025）09-4422-10

Effects of Plasma Steroid Hormones on Superovulation Efficiency in Tan Sheep afterSuperovulationTreatment

XING Zhou， SONG Chenglei， CAO Fengfeng，LI Zhuoying，LI Qingyun， TAO Jinzhong*（College of Animal Science and Technology， Ningxia University， Yinchuan 75oo21， China）

Abstract： This study aimed to explore the relationship between changes in plasma steroid hormones and superovulation effects in Tan sheep after superovulation treatment. Thirty-three healthy multiparous Tan sheep aged 4.5±0.1 years were subjected to superovulation using the CIDR+FSH+PG method. Blood samples were collected on the day of artificial insemination （AI，Day O） and embryo collection（Day 6），and plasma was separated. Plasma samples were divided into 4 groups based on the number of ovarian corpora lutea observed on the embryo collection day and the sampling time： high-response group on AI day（HCA），low-response group on AI day（LCA），high-response group on embryo collection day（HC6），and lowresponse group on embryo collection day （LC6），with 8 samples in each group. Liquid chromatography-tandem mass spectrometry （LC-MS/MS） was used to target and detect changes in plasma steroid hormones. The results showed that progesterone（P4），pregnanediol （PdG）， deoxycorticosterone （DOC） and 2-methoxyestrone） （2-ME） in plasma of Tan sheep on embryo collection day were significantly higher than those on the day of AI，while 4-methoxyestrone （4- ME） in plasma on the day of AI （LCA and HCA groups） was significantly higher than that on the day of egg collection；The concentration of 17α -hydroxyprogesterone in the HCA group （ FC=1.50 ） was significantly higher than that in the LCA group （ ⋅P<0. 05 .The concentrations of progesterone （ FC=0.52 ）and deoxycorticosterone（ FC=0.50 ）in the HC6 group were significantly higher than those in the LC6 group （ ⋅P<0. 05 ．Additionally，the concentrations of progesterone and deoxycorticosterone on the embryo collection day （6 d） showed a strong correlation with the number of corpora lutea and the total number of embryos. The results indicate that after superovulation treatment，plasma steroid hormones in Tan sheep change，and the concentrations of progesterone and deoxycorticosterone affect the superovulation effect on the embryo collection day.

Keywords： Tan sheep；superovulation；corpus luteum number;steroid hormone x Corresponding author： TAO Jinzhong，E-mail ：tao_jz@nxu. edu. cn

自上世纪90年代以来，超数排卵与胚胎移植技术（multiple ovulation and embryotransfer，MOET）被广泛应用于绵羊的扩繁和育种行业，但超排效率仍然受到供体排卵率等多方面因素的影响。（剩余15816字）