马卵母细胞玻璃化冷冻保存研究进展

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中图分类号：S821.35 文献标志码：A 文章编号：0366-6964（2025）09-4143-13

Progresson Vitrification Cryopreservation Oocytes

YANG Mingying，WANG Na， LIU Yuanyi， LI Xinyu， Bayanamar， SHI Yujie，MANG Lai， DU Ming*

， Germ plasm Affairs，College Animal s， Agricultural University，Hohhot Olool8，China）

Abstract：Oocyte cryopreservation is significant importance for human assisted reproductive technology livestock production.This technology aids in the conservation genetic resources from superior breeds the maintenance biodiversity. However，due to their small surface area-to-volume ratio low membrane permeability，oocytes are highly susceptible to damage during freezing.This includes mechanical injury caused by ice crystal formation，oxidative stress， the toxicity cryoprotectants，all which severely reduce their developmental competence after thawing. Compared to other animals，the high lipid content within equine oocytes reduces their cryotolerance，making cryopreservation considerably more challenging. Although fspring can be obtained from vitrified immature oocytes，the developmental competence vitrifiedthawed oocytes remains to be enhanced compared to their fresh oocytes.This article primarily reviews the types damage caused by vitrification to equine oocytes the factors influencing the cryopreservation efficiency equine oocytes. It aims to provide a reference for further establishing optimizing vitrification protocols for equine oocyte cryopreservation.

words： equine;oocytes;vitrification;cryodamage;cryoprotective agent ∗ Corresponding author：DU Ming，E-mail ：duming@imau. edu.cn

配子和胚胎的低温保存技术作为辅助生殖领域的重要一环，已在家畜繁殖中得到了广泛应用。（剩余23484字）