牛FOXP2基因的单等位基因表达和DNA甲基化状态分析
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中图分类号：S823.2；S813.3 文献标志码：A 文章编号：0366-6964（2025）09-4369-10
Analysis of Monoallelic Expression and DNA Methylation Status of Bovine FOXP2 Gene
ZHENG Yunchang1， Ruilin1， Xiaohe'， Lidan¹， Yinjiao1 ， Haonan1， Weina²， Cui1，LI Shijie1*
（1. College of Life Science， Hebei Agricultural University ， Baoding O71ool，China ;2.CollegeofMedical Science，Hebei University，Baoding O7looo，China）
Abstract： The aim of this study was to investigate the monoallelic expressions and DNA methylation status of the forkhead box P2 （FOXP2） gene in bovine tissues and placentas. The bovine tissues of two growth periods （calves of O d old and adult cows of 4-5 years old） and fullterm placentas naturally delivered were collected. The expression levels of the FOXP2 gene were analyzed in bovine6 tissues （heart，liver，spleen，lung，kidney and brain）and placentas by qRTPCR. The monoallelic expressions of the FOXP2 gene were analyzed using the method of direct sequencing of RT-PCR products based on the single nucleotide polymorphism （SNP）. The analysis of DNA methylation status in bovine tissues， placentas and sperms was conducted using sulphite sequencing method. The results showed that the expression of FOXP2 gene in all 6 tissues （heart，liver，spleen，lung，kidney and brain） of adult bovine was higher than that of calf tissues. Based on the SNP site of g.53771685G>A situated in the 3′ non-coding region of the FOXP2 gene，monoallelic expression of FOXP2 was observed in 6 tissues of calves. However， in adult cattle， FOXP2 showed monoallelic expression only in brain tissue and biallelic expression in other 5 tissues （heart，liver，spleen，lung and kidney）. The FOXP2 gene was mathernally expressed in the placenta. Hypomethylation was showed at the promoter and the 5′ end of FOXP2X1 splice in bovine tissues，placentas and sperms. These results indicated that the FOXP2 gene is an imprinted gene and the maternal allele is expressed in bovine placenta， and the monoallelic expression inbovine tissuespresentsgrowth period-and tissue-specific. Hypomethylation is in the promoter and 5′ regions of FOXP2 gene，indicating that DNA methylation is not involved in the regulating monoallelic expression of bovine FOXP2 gene.
Keywords： forkhead box P2（FOXP2） gene； allelic expression;DNA methylation; bovine∗ Corresponding author： LI Shijie，E-mail： lishijie20oo5@163.com
在哺乳动物发育过程中，由于同源染色体上表观遗传修饰不同而导致亲本等位基因表达不同，其中来源于一条亲本的等位基因不表达或表达水平很低，只有一条亲本的等位基因表达的现象，称为基因组印记（genomic imprinting）[1-2]。（剩余16048字）
