6种病毒复合侵染“玛莎莉”甘薯的鉴定和分析

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中图分类号S435.31文献标识码A文章编号 0517-6611（2025）12-0141-04

doi：10.3969/j.issn.0517-6611.2025.12.032

开放科学（资源服务）标识码（OSID）：

Identification and Analysisof a Mixed Infection of Six Viruses in Sweet Potato Cultivar“Mashali"

ZHANG Xiao-yan¹，LIUYu-shan’，ZHU Wen-l²etal（1.Shandong（Linyi）InstituteofModerAgriculture，ZhejiangUnivesiy，iiyi，Shandong 276OoO;2.Linyi Vocational University of Science and Technology，Linyi，Shandong 276034）

AbstractTranscriptomesequencingandRT-PCRwereperformedonfourswetpotatosamplescolectedfromLinyiCityShandongProvince，tostudyttysofusttfeedswpotato“Mashl，noertrovidfercforhdigoffotato sedlingsandthefoulationofprevetionandcotroltrategies.Theseetpotatosamplesof“ashali”showdobviousosaicndcrikling symptoms.Sixknoviuses，incdingsweepotatofatheryotlevirus（PFV），swetpotatolatenvius（SPLV），sweepoatoeafcurl virus（SPLCV），sweetpotatovirusG（SPVG），sweetpotatovirusC（SPVC）andsweetpotatovirus2（SPV2）wereidentified.Thspecific primersoftheaboveviruseswereseletedforRPCRdetectio，tetargeandsiththeexpectedsizeofthesixviuseswereobaidand thetargetviussequences wereverifdbysequencing.TeresultsofRT-CRandtranscriptomesequencingwereconsistentwittesulsf 3samplescontaining6virusesand1samplecontaining4viruses.TephylogeneticanalysisbasedontheCPgeneshowedthattheetected SPFMV hadacloseevolutionalrelationshipwithSPFMVrusetcrack（RC），thedetectedSPLCV，SPV2andSPVCwere mostcloselyrelated totheisolatesofina，PVGasostoelyelatedtoholatesofCndtheeresiantfrecsogisolatefroV colectefrodftgiouloftsdyillprvdealsisforstablstofldotroldvfr nology system of sweet potato cultivar“Marasali”.

Keywords Sweet potato virus disease;Mixed infection;Transcriptome;RT-PCR

甘薯[Ipomeabatatas（L.）Lam]是集饲料、粮食和工业原料于一体的多用途作物，是我国种植面积较大的作物之一。（剩余8280字）