高产虾青素红法夫酵母发酵工艺及培养基优化

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中图分类号TS202.3文献标识码A文章编号 0517-6611（2025）12-0129-04

doi：10.3969/j.issn.0517-6611.2025.12.029

开放科学（资源服务）标识码（OSID）：

Fermentation Process and Culture Medium Optimization for High Astaxanthin Producing by Phaffia rhodozyma ZHU Chao-yang，TIAN Shao-yang，LI Hui-ying etal（Luoyang Chihonbiotechnology Co.，Ltd.，Luoyang，Henan 47100

AbstractObjective]TostudytheefectsoffermentationconditionsandmedumfomulationontheproductionofastaxanthinbyPaffa rhodozma.MethdIntiallyinglefctoxpeintseoducedtvestigatettsofliiotempeaturendu quently，eachpotisiceatiadiiallstedoghglectxpetsialli tionalcomponentswreaddedorremovedfrothebasicfermentationmedim，andachodfcationwastestedseparately.Resultftera 120h fermentation process，the biomass increased from 22.0 to 38.4g/L ，while the astaxanthin yield rose from 38.5 to 150.1mg/L. [Conclusion]The optimal cultivation condition is 20% ， pH5.0.A nd the optimal medium formula is glucose 30.0g/L ， yeast extract 3.0g/L ，peptone 1.0g/L ， hydrolyzed soybean meal 4.5g/L ，corn steep powder 3.0g/L ，ammonium sulfate 5.5g/L ，MgSO4 ·7H2O 0.1g/L ;the cultivation time is 120h ：

Key wordsAstaxanthin; Phafa rhodozyma ; Culture medium optimization;Fermentation process optimization

虾青素（Astaxanthin）是一种类胡萝卜素[1]，化学名称为3，3′ -二羟基- ⋅β，β′- 胡萝卜素 -4，4′ -二酮，分子式为 C40H52 04[2] ，其广泛存在于甲壳纲动物（如虾、蟹）等、鱼类（如鲑鱼、红鳟鱼）及鸟类（如火烈鸟、红鹦和火鸡等）中[3]。（剩余8454字）