梵净山石斛林下仿野生抚育试验及其多糖和甘露糖含量测定

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中图分类号S567. 23+9 （20 文献标识码A 文章编号 0517-6611（2025）11-0167-05

d oi;10.3969/j . issn. 0517-6611.2025.11. 036

开放科学（资源服务）标识码（OSID）：

Experimental StudyonImitation WildTendingof Dendrobium fanjingshanenseintheForestandDeterminationofContentsof Its Polysaccharide and Mannose

CHENG Yun1 ，WANG Hai1，TANShan-cai² etal（1.Tongren Agricultural andRural Bureau，Tongren，Guizhou 5543O0;2.Tongren Poly technic College，Tongren，Guizhou 554300）

AbstractObjetie]TostudythegrowthcharacteriticsofDendrobmfanjngshanensefromimitationwildtendingintheforestanddeter minethecontentofplsaccharideandmaose.MethodUsinghangingots，achedtresandatachedstonsuderteforsttosilate thewildabitatgothofndrobfjgshestntentofolahadsiDendrobmfjgsheseasdtdbol sulfuricacidmetho，ndthemaoseontentwasdeterinedbyprecolunderivatizationultra-highpeforanceliquidhoatgaphy （UPLC）.ResultTheeight，freshweightanddryweightofDendrobmfajingshnensegrowninhangingpotsundertheforestweresignificantly higher than those grown in attached stones and trees （ P<0.05 ）. The polysaccharide and mannose contents of Dendrobium fanjingshanense grown in hanging pots were 20.41% and 14.23% ，respectively. Compared with Dendrobium fanjingshanense grown in attached stones andtrs，thepolysaccarideandmaosecontentsofDendrobiumfanjingshanensegrownihangingpotsweresignificantlydiernt（ P< 0.05）.[Conclusion]Hangingpotsundertheforestforurturingisanfeiewaytoiitatonwildtendingandcultivatehigualityand high-yielding of Dendrobium fanjingshanense.

Key wordsImitation wild tending;Dendrobium fanjingshanense;Growth characteristics;Polysaccharide;Mannose

梵净山石斛（Dendrobium fanjingshanense Z.H. Tsi ex X.H.JinetY.W.Zhang）属于兰科（Orchidaceae）石斛属（Den-drobium）多年附生草本植物，叶生于茎的上部，先端稍钝、稍有钩转，花苞片在基部或近中部具有一赤褐色斑块，花黄色，花被片反卷，为石斛属特征明显的物种[1，是2001年在贵州省梵净山海拔 800～1450m 的高大树枝上以及山谷和林缘的岩石上发现的新种[2]，2010年在浙江省遂昌九龙山发现有少量分布，附生于海拔 500～700m 阔叶林中的毛红椿树上或山体岩石上[3]。（剩余10530字）