PbCYP86A1/B1调控梨果皮褐色形成的机制研究

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中图分类号 S661.2 文献标识码A

文章编号 0517-6611（2025）11-0079-07

doi：10.3969/j.issn.0517-6611.2025.11.018

开放科学（资源服务）标识码（OSID）：

Study on theMechanism of PbCYP86A1/B1 Regulating the Formation of PearPeel Brown Color

LIFei，JIANGPing，HNGWei（1.ChzhouAgriculturalandRuralTechnologyCnter，Chuzhou，Anui2390O;2.AnuiAgiculural

University，Hefei，Anhui ） AbstractObjetie]ThereltioshipbetweenPCYP86A1，PCY86B1genesandpearplbrowntraitswasexplored.MethodThePbCYP86A1/B1genewasclonedfromthepelof“Dangshansu”pearand“Dangshanjinsu”pearbyreverse ranscriptionpolymerasechainreaction（RT-PCR）.Thevirus-inducedgenesilencing（VIGS）methodwasusedtosilencethetargetgeneusingthetobacocrispycrackvirus （TRV）vector.Byanalyzing thecontrol group（TRV2-TRV1）andthe experimental group（TRV-PbCYP86A1，TRV-PbCYP86B1）pearpericarppheotyptomaticatiolatiexpressonoftgenedangsinompositioftesberin.Resultsuls showedthatafterTRV-PbCYP86AlandTV-PbCYP86Blwereinjectedintothepearepidemis，suberinintheinjectionpartscouldnotbe accumulatedwittfrutdvelopntadidihelowgreetcoldierebeweeneijeonsitendesurronger icarpwassignificantlygreatertantatoftecontrloup.-PCRidcatedthatthxpressosofYP86A1andPCYP86Bgeesre ducedsignfcantlywithsignifcantdierenceintheexocarof‘Xiusu’pearwithVIGScomparedihontrol.Conlusion]eresultsofsuberincontentanalysisshowdthatbothPCP86AlandPCYP86BgensilencingcouldsignificantlyihibithsynthesisofC6doy fatty acids and -diacid in pear pericarp，which will reduce the total amount of suberin.

Key WordsPear; PbCYP86A1 gene; PbCYP86B1 gene;Suberin; Virus induced gene silencing

“砀山酥梨”（Pyrusbretschneidericv.‘Dangshansuli’），属于蔷薇科白梨系统，黄绿色表皮，但其芽变品系“砀山金酥”呈褐色，通过分子标记确定为遗传性变异[1]。（剩余14893字）