超声辅助酶法提取桦褐孔菌多糖的工艺优化及其对 α -葡萄糖苷酶的抑制活性

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中图分类号：S646 文献标志码：A 文章编号：1671-4652（2025）02-0110-07

DOI：10.16872/j.cnki.1671-4652.2025.02.014

ABSTRACT：This studyaimed tooptimize theultrasonic-assted enzymatic extraction processof Inonotus obliquus poly saccharides and evaluate their inhibitory activity against α glucosidase. Single-factor experiments were conducted to identify key factors afecting the polysaccharide extractionrate，including enzyme dosage，liquid-to-solidratio，ultrasonic temperature andultrasonic time.Orthogonal tests were then used tooptimize theextractionconditions，which revealed that the optimal conditions were an enzyme dosage of 1% ，a solid-to-liquid ratio of 1：20 ，an ultrasonic temperature of 50∘C and an ultrasonic time of 20min .Under these conditions，the extraction rate of I .obliquus polysaccharides reached 4.97% . Furthermore， I.obliquus polysaccharides demonstrated significant inhibitory effects on α glucosidase，with an IC50 value of 0.296mg⋅mL-1 ，indicating a potent α glucosidase inhibitory activity and its potential application in blood sugar-lowering therapies.

KEY WORDs： Inonotus obliquus； polysaccharides；ultrasound-asisted enzyme extraction;orthogonal experimental optimization; α glucosidase

桦褐孔菌（Inonotus obliquus）是一种药食两用真菌，自十六七世纪以来在俄罗斯民间传统医学中得到广泛应用[1-2]。（剩余11490字）