新型鹅星状病毒基因1型和2型双重荧光定量PCR方法的建立及应用

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中图分类号： S852.65+9.1 文献标识码：A文章编码：1005-8567（2025）04-0083-11

Abstract：In order to develop a credible dual fluorescence quantitative polymerase chain reaction （qPCR） for detecting novel goose astrovirus type1（NGAstV-1）and type2（NGAstV-2），primers were designed to establish a dual fluorescenceqPCR detection method using ORF2 proteinof NGAstV-1and NGAstV-2，and36samplesof goose liverand kidneytisuesuspectedtobeinfectedwithNGAstV-1andNGAstV-2 were evaluated inthis study.The test results showed thatthe correlation coeffcients of qPCR were O.997and were negative for novel duck reovirus （NDRV），avian influenza virus （AIV），fowl adenovirus（FAdV），and goose parvovirus（GPV）.The minimum detection limit was 1.12×101copies/μL .Thereproducibility test results indicate good reproducibility，witha coefficient of variation between groups of less than 0.8% andacoefficient of variationwithin groups of less than 0.5% It canbeseenthattheqPCRcan eficientlyandspecificalldetect NGAstV-1and NGAstV-2，providingafeasible method for NGAstV monitoring.

Keywords： Novel goose astrovirus;qPCR;ORF2

雏鹅痛风是由新型鹅星状病毒（NovelGooseAstrovirus，NGAstV）引起的一种以关节肿胀、无法站立、关节腔内和肾脏等内脏器官出现大量尿酸盐沉积的病毒性传染病[1-7]。（剩余12642字）