玫瑰精油及活性成分对海豚链球菌等3种水产源细菌的抑制作用研究

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中图分类号：Q946.85 文献标识码：A 文章编码：1005-8567（2025）04-0045-06

Abstract：To experimentally investigate thein vitro inhibitory zonesizeand minimum inhibitory concentrationof roseessentialoilanditsantimicrobialactiveingredientscitronelol，geraniolandnerolagainstthreeaquatic pathogens，namelyVibrioparahaemolyticus（V.parahaemolyticu），Streptococcus iniae（S.iniae），ndVibrio harveyi（V. harveyi）.Theinhibitoryefectsofroseesentialoilanditsntibacterialcomponents（citronellol，geraniolandnerol） onthreeaquaticpathogenic bacteria（V.parahaemolyticu，S.iniae andV.harveyi）was tested.The diameter of the inhibitionzonesofroseesentialoilanditsantibacterialagents wasdeterminedusingthemethodsof punch-holeand cross-validation.Theminimuminhibitoryconcentrations（MIC）ofroseessentialoiland itsantibacterialcompounds weredeterminedusing the96-wellplate microdilution method.Finally，the fractionalinhibitoryconcentration（FIC） indicesof thethreeactive ingredients weredeterminedusing the micro-broth chessoard cross-validationmethod.The resultsdemonstrated thatrose esentialoilanditsactiveingredientsexhibitedantibacterialactivityagainst V. parahaemolyticu，S.iniaeand V.harveyi.Among these，citroneloldemonstrated the most pronounced antibacterial efect.The threerose essentialoilactive ingredients were tested incombinationwithoneanothertoevaluate their joint antibacterial efect，withthefindingsindicating thatthecombinedefectwaseitherirrelevantorantagonistic.Rose essentialoilanditsactive ingredients have been demonstrated toexertnotablebacteriostatic efectsonaquatic pathogenicbacteria.However，the simultaneousapplication of itsactiveingredients ina clinical setting isnot recommended.Thisstudywillprovideatheoreticalfoundationforthefutureapplicationof theantibacterialeffectsof rose essential oil in aquaculture.

Keywords：Roseessential oil；Antibacterial efect；Combinedantibacterial efect；Vibrioparahaemolyticus; treptococcus iniae;Vibrioharveyi

我国是水产养殖大国，随着水产养殖规模与集约化养殖的日益发展壮大，生存环境恶化，水产动物疾病也向多发、复杂状态发展。（剩余6729字）