米林藏鸡禽白血病的监测与分析

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关键词：米林藏鸡；禽白血病；PCR核酸检测； p27 抗原ELISA检测中图分类号： S854.4+3 文献标识码：A文章编码：1005-8567（2025）04-0040-05

Abstract：To explore the detection effctofdiferent detection methodsand the feasibilityof thecombinationof avian leukemia virus（ALV） infection andAL purification，the study in chicken，using ALV p27 antigen ELISA methodandALV-JPCR nucleicacid detection method，from2020to 2024 atotalof1333randomlycollctedwhole blood samples forALVdetection analysis.The results showedthat in Tibetan chickens，thepositiverate of ALV p27 antigen ELISA testwas 15.42% ，ALV-JPCR nucleic acid test was 2.49 % .The total positive rate of the two detection methods was 17.23% ，among which only 3 individuals were positive for the two detection methods，accounting for （204号 3.95% of the total positive individuals.In addition，sinceAL purification in 2O2O to 2O24，theAL positive rate of milinTibetan chickensdecreased from 17.23% to Obetween 2O2O and 2024. The results show that there are mixed infectionofALV-Jsubgroupandothersubgroups intheflock；thecombinationofthe two methodscangreatlyimprove theALVdetectionrateandcan providereferencefor theactual purification work；theALpurificationofmilin Tibetan chickengroup issignificant，which basicallrealizes theeffectivecontrolofALintheflock，whichcanprovidedata support for the AL prevention and control purification of this species.

Keywords：Milin Tibetan chicken；Avian leukosis；PCR nucleic acid test;p27antigen ELISA tes

禽白血病（AvianLeukosis，AL）是由禽白血病病毒（AvianLeukosisVirus，ALV）引起的一种免疫抑制性、种源性传染病，具有水平和垂直传播特点[1]。（剩余6459字）