没食子酸改性氧化石墨烯光热治疗微针的制备与评价

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Preparation and evaluation of gallic acid-modified graphene oxide microneedles for photothermal therapy

Feng Yifan，Jin Wei，Li Ying**，Dong Zhengqi StateKeyLaboratoryfQualitynsuranceandSustaiableUseofaodiHerbs，IstiuteofMedicinalPantDevelopentChinecadey of Medical Sciences，Peking Union Medical College，Beijing10o193，China **Corresponding author：LiYing，email：yli@implad.ac.cn；Dong Zhengqi，email：zqdong@ implad.ac.cn

[Abstract]ObjectiveToprepareaphotothermal therapeuticmicronedleforthe treatmentof age-related chloasmathatinhibitsmelaninsecretion，andtoevaluateitsperformanceandeficacyMethodsGAGOnanocompositeswerepreparedbymodifying thegrapeneoxide（GO）structure withgalicacid（GA）；thesynthesisofGAGOwasverifiedusing UV-Visspectrophotometryand Fouriertransform infraredspectroscopy；thestabityofGOandGAGO inaqueoussolution，PBSandRPM1640 medium wasdetected usingaMalvemparticlesizer；three matrix materials，hitosan，poly-vinypyolidone（PVP），andpolyinylalcohol，wereeed andthe micromolding perfusionmethodwasdetermined.Their topographywascharacterizedusing ightmicroscopyandscaning electronmicroscoy；mechanicalpropertiesofmicrondlesweretestedusinganIstrostroncompresio-tesiontester；skinsertion propertiesofmicroneedlesweretestedusing SDratskin；photothermalconversionpropertiesof microneedleswereasessedbyan 808nmnear-infrared（NIR）laser；andtheinvitroinhibitoryefectofGAGOphotothermal therapywasverifiedusingB16melanoma cels.ResultsGAwassuccessullyloadedonGOandhad goodstabilityandphysicochemicalproperties.GAGO-PVPmicroeedles weresuesfullyconstructed，withbeautifulappearance，easypouringanddemoulding，intactneedetipswithoutbubbles，and throughmechanicalpropertistesting，telossofficacyreached3.66Nneede，hichanpeneatetesinwitoutcausigla mation，andhasgoodphototheralconversionproperties.Comparedwiththecontrol group，GAGOcouldsignificantlyincreasetheac tivityofB16melanomacell，ecreasetyrosinaseactivityandinbitmelaninseretionduringNRchallnge，andtedierecsere statistically significant（ P<0.001 ）.Conclusion GAGO NIR treatment significantly inhibited B16 melanoma cell viability and melanin secretion，and GAGO-PVP microneedles have the potential to be applied in melasma treatment.

[Key words] Graphene oxide；Gallic acid；Melasma；Microneedle；Photothermal therapy

黄褐斑是皮肤科常见病、多发病及难治病，主要成因是紫外线暴露、家族史、怀孕和使用外源性激素等，多见于中老年女性，外治法是黄褐斑的常用治疗手段[1]。（剩余12182字）