基于秀丽隐杆线虫模型的林下山参抗衰老作用研究

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[Abstract]ObjectiveToevaluatetheanti-aging efectsofmountain-cultivated ginseng using the Caenorhabditiselegans modelandpreliminarilyinvestigateitsmechanismsthroughetworkpharmacologyanalysisandR-qPCRexperiments.MethodsThe studyemployedCaenorhabditiselegansasanagingmodelorganism.Theimpactofthewaterextractofmountain-cultivatedginsegon thethermotoleranceofN2wild-tyeCaenorhabditiseleganswasobservedusingaeatstresschalengeassayAging-relatedphyiological indicators weremeasured，ncludinglifespan，bodylength，locomotorabity，superoxidedismutase（SOD）andmalondialdehyde （MDA）levels，lipofuscinaccumulation，andreactiveoxygenspecies（ROS）levels.GeneOntologyfunctionalenrichmentanalysisnd Kyoto Encyclopediaof Genes andGenomes pathway enrichmentanalysis wereperformedbasedonnetwork pharmacology.The“mountain-cultivatedgnhcalopontssectiogesigsialingatas”okaosuctdpe naryvalidationofpotentialsignalingpathwaysviauantitativereal-timepolymerasechanreacton（RTqPCR）ResultsThewaterextracof mountain-culivatedGinsengsignificantlextendedCaenohabditiseleganslifespan，ncreasedsurvivalatesunderhats， enhancedbodylengthandlocomotoractivitylevatedSODlevels，andreducedaccumulationsofMDA，ipofuscinandO，andte differences were statistically significant （ P<0.05 ）.Network pharmacological analysis indicates that biological processes exhibit a significantenrichmentof targets；mountaincultivatedginsengayexertitsanti-agigpharmacologicalffectsbymodulatingultiplesig naling pathways，includingPI3K-AKT，Ras，andHF-1signalingpathways.RT-qPCRresultsconfirmedmountain-cultivatedginseng significantlydowgulatedthmRAexpresioofge-1，t-1andf-，hilemarkedlyprgulatigdf-1eion levels（genesasociatedwiththePI3K-AKTsignalingpathwayinCaenorhabdiselegans）ConclusionMountain-cultivatedginseng efectivelydelaysaging in Caenorhabditis elegans，primarilythrough suppressionofthe PI3K-AKTsignalingpathway. [KeyWords]Mountain-cultivated ginseng；Anti-aging；Caenorhabditis elegans；Network pharmacology

我国人口老龄化趋势日益严峻。（剩余11523字）