缺硼胁迫对油茶生理及硼转运基因表达的影响

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中图分类号：S722 文献标志码：A 文章编号：1000-2006（2026）02-0119-10

Abstract：【Objective】Thisstudyaims to screen boron（B）deficiencytolerant Camelliaoleifera cultivars，analyze the physiology of B deficiency tolerance and the molecular mechanism of B transport in C .oleifera.【Method】The physiological responses of seven c . oleifera cultivars seedlings underB deficiency stress were investigated.The affiliation functionvalue methodwasused to evaluate theresistanceof diferent cultivars toB deficiency.Andthe tolerance differences between B-efficient and B-inefficient C .oleifera cultivars from the perspective of four B transporter gene expression were analyzed.【Result】（1）Under the B deficiency stress environment，the symptoms of seven cultivars were yellowing and curling ofleaves，browningand shedding ofroots，and stunting of growth.（2）Bdeficiency stressreduced the net photosynthetic rate and other photosynthetic parameters，and the B content in some C .oleifera cultivarsdecreased significantly.TheBdeficiencyreducedthecontentandactivityofosmoticregulators suchassolublesugars，hydrogen peroxide and superoxide anions in leaves and rots，as wellassuperoxide dismutase.（3）Theresultsof membership functionanalysis showed that‘Changlin 4O’and‘Changlin 4’were more tolerant toBdeficiency，while‘Huajin’was the leasttolerant toB deficiency.（4）The expresions offourB transporter genes（CoBOR1，CoBORl-like，CoBOR2， andCoNIP5;1）in‘Changlin 4’and‘Huajin’were significantlyup-regulated，andthe expresson levelsof the four genesin theroots and leavesof the B-deficiency-tolerantcultivar‘Changlin4’were significantly higherthan thoseinthe B-deficiency-tolerant cultivar‘Huajin’.【Conclusion】The tolerance of diferent C .oleifera cultivars to B deficiency stress varies greatly.The B-effcient cultivars could enhance their abilityof boron deficiency tolerance byup-regulating theexpression of B transportgenes，improving photosynthetic eficiency，antioxidantcapacityand osmoticregulation capacity.

Keywords：Camellia oleifera；borondeficiencystress；photosynthetic effciency；antioxidant enzymes；boron transporter genes

硼是植物正常生长发育必需的微量元素之一，在植物细胞壁组成、细胞膜稳定、光合产物运输以及花器官发育等方面均具有重要作用[1]。（剩余17667字）