三化汤的化学成分、人血原型成分及代谢成分分析

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中图分类号 R917;R284.1 文献标志码A 文章编号 1001-0408（2025）14-1760-05

DOI 10.6039/j.issn.1001-0408.2025.14.12

ABSTRACTOBJECTIVETo studythechemical components，components migrating tothe bloodand metabolitesof Sanhua decoctioninrats.METHODS Male Wistarrats were divided intoadministration groupand blank group，with 6rats in each group. The rats in the administration group were given 13.3g/kg of Sanhua decoction lyophilized powder solution by gavage once a day in themorningandevening，and theratsinthe blank group weregivenanequalvolumeofsalinebygavageonceadayinthe morningand evening，both for3consecutivedays.Plasmasampleswerecolectedfromthetwogroupsofratsafterthe last administration.Thechemicalcomponents，prototypecomponentsmigrating tothe bloodand metabolitesof Sanhuadecoctionwere analyzedbyUHPLC-Q-TOF/MStechnique.Thestructureswereidentifiedcombinedwiththeself-builtnaturalproducthighresolutiomassspectrometrydatabaseandrelevant literature.RESULTS&CONCLUSIONSTotally69compounds wereidentified fromtheyophilizedpowderofSanuadecoction，incuding29componentsfromRheumoffcinale，16componentsfromMagnoliae OfficinalisCortex，22components from AurantiiFructus Immaturus，and1Ocomponents from NotopterygiihizomaetRadix. Amongthem，3components（citricacid，L-tyrosine，adenosine）werepresentinall4medicinal herbs.Anothercomponent （feruloylgluconicacid）stillneededtobespecificallyaributedAtotalof43prototyecomponentsmgratingtoteloodwere identified，incldingfavonoids，peols，ntaqnones，penylpropanoids，lkaloidsandtriterpeois.Atotalof6ablites wereidentified，predominantlyconsitingofflavonoids，anthraquinonesandphenylpropanoids.Themetabolicpathwaysmainly involved phaseImetabolicreactions such as demethylation and phase I metabolic reactions like sulfation and glucuronidation.

KEYWORDSSanhua decoction；chemicalcomponents；prototypecomponents migrating tothe blood；metabolites；UHPLC-Q TOF/MS technique

三化汤出自金代刘完素《素问病机气宜保命集》，由厚朴、大黄、枳实、羌活组方而成，是2018年国家中医药管理局公布的《古代经典名方目录（第一批）》中的名方之一。（剩余8194字）