指纹图谱结合药效成分含量综合评价银丹活血通瘀颗粒质量

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中图分类号R917 文献标志码A 文章编号 1001-0408（2025）14-1743-06

DOI 10.6039/j.issn.1001-0408.2025.14.09

ABSTRACTOBJECTIVEToevaluate thequalityof Yindan huoxue tongyu granules.METHODS Taking high performance liquidchromatographywithultravioletandevaporativelightscateringdetectionasmethod，thefingerprintof15batchesofYindan huoxuetongyugranuleswasestablished，andsimilarityevaluationwasperformedby SimilarityEvaluationSystemof ChromatographicFingerprintofTCM（2012edition）todeterminecommonpeaks.Thecommonpeakswere identified by comparingwithreferencesubstancechromatogramsandsingledecoctionpiecechromatograms.Networkpharmacologywasusedto screenoutcoretargetsandpathwaysof identifiedcomponents，constructa“component-target-pathway”network diagram，and predictthe pharmacodynamiccomponentsof Yindan huoxuetongyu granules，and the content determinationof these components wascarriedoutbythe samemethod.RESULTSHPLC fingerprintsof15 batchesof Yindanhuoxue tongyugranules were characterized with 40 common peaks，and17components including salvianolic acid B，astragaloside ，notoginsenoside R1 ，and ginkgolideAwereidentified.Network pharmacologypredictedthat17componentsmainlyactedon97coretargetsand137 pathways toexerttheirpharmacologicalefect.Averagecontentsof13bioactivecomponentsin15batchesofsampleswere068， 0.2320，0.0738，0.3532，3.6202，0.1910，0.3333，0.3174，0.7850，0.5382，0.4600，2.475 1 and 0.3477mg/g ，including calycosin-7-O- β -D-glucoside，rosmarinic acid，formononetin，lithospermic acid，salvianolic acidB，ononin，ginsenoside Rb1 ， ginsenoside Rd，ginkgolide C，ginkgolide A，ginkgolide B，notoginsenoside R1 ，and astragaloside . CONCLUSIONS The establishedfingerprintofYindanhuoxuetongyugranulescanreflecttheoverallcharacteristicsofthepreparation.Thecontent determinationmethodforitspharmacodynamiccomponents，developed incombinationwithnetworkpharmacology，isaccurate， reliable，and exhibits good repeatability，making it suitable for evaluating the qualityof Yindan huoxue tongyu granules.

KEYWORDsYindanhuoxue tongyu granules；fingerprint；contentdetermination；network pharmacology；pharmacodynamic components；quality evaluation

银丹活血通方是由黄芪、丹参、三七、银杏叶4味中药组成的临床经验方，具有活血化瘀、补气行血之功效，临床主要用于缺血性脑卒中（ischemicstroke，IS）的治疗，疗效显著。（剩余10627字）