参芪固本方调控IL-17信号通路改善癌因性疲乏的作用机制

打开文本图片集

中图分类号R965；R285 文献标志码A 文章编号 1001-0408（2025）14-1722-08

DOI 10.6039/j.issn.1001-0408.2025.14.06

ABSTRACTOBJECTIVEToexplore the mechanismof Shenqi guben formula（SQGB）in improving cancer-related fatigue （CRF）based on network pharmacologyand celular experiments.METHODS Activecomponentsof SQGB and CRF-related targetswereidentifiedonthebasisofdatabasessuchastheTraditionalChineseMedicineSystemsPharmacologyplatform.Anin vitro CRFcellmodel was establishedby inducing A549celswith interleukin-17（IL-17）.Cells were treated with low（ 1.0mg/mL ）orhigh （1.5mg/mL ）concentrations of SQGB.The effectson cellviability，migration，apoptosis，inflammatoryfactors， mRNA expression，apoptosis-related proteins and key proteins of IL-17 signaling pathway were evaluated using scratch assay， flowcytometry，ELISA，real-time fluorescent quantitative PCR and Western blot analysis.RESULTS SQGB contained 84 active components acting on 209 potential CRF targets. Among these，quercetin，kaempferol，and luteolin were identified as thecore components of the compound.Core targets included tumorproteip53，AKTserie/threoninekinase1，I-6，andtumornecrosisfactor（TNF）IL-17，TFandphosphatidylioitol-- kinase-serine/threonineprotein kinase（PI3K/Akt）signaling pathwayswereidentifiedascrucial pathways.Compared with IL-17 interventiongroup，thecellmigrationrate，B-cellymphoma 2（Bcl-2）protein expression，thelevelsofIL-6and TNF- α in the supernatant，mRNA expression of IL-17 receptor A（IL-17RA），TNF- α⋅∝ ，and IL-6，as well as the protein expression of IL-17RA and nuclear factorkappa-Bp65subunit（p65），andphosphorylated（p）-p65/p65ratioinIL-17+SQGBlow-andhigh-quality concentration groups were all significantly decreased or down-regulation （P<0.05 ）；theapoptosis rate，expression levels of Bcl-2 asociatedXprotein（Bax）andcleavedcaspase-3protein，theratioofBax/Bcl-2，theexpresionlevelofp-p38protein，andthepp38/p38 ratio were all significantly increased or up-regulated（ P<0.05 ）.Moreover，the improvement effects of these indicators weremostly beterinthehigh-quality concentrationgroups compared tothelow-qualityconcentrationgroups（ P<0.05） 1 CONCLUSIONSSQGBameliorates CRFbyregulating the IL-17signalingpathway，inhibiting the expresionof inflammatory factors，and activating p38 MAPK-dependent apoptosis pathway.

KEYWORDsShenqi guben formula；network pharmacology；cancer-related fatigue；celllar experiments

癌因性疲乏（cancer-relatedfatigue，CRF）被定义为持续存在的、与活动量不匹配的疲乏感，是恶性肿瘤患者的常见症状之一。（剩余15213字）